Construct: ORF TRCN0000474278
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006314.1_s317c1
- Derived from:
- ccsbBroadEn_14307
- DNA Barcode:
- GGGCAAAGCCCTTCATGATTTGAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- FAR1 (84188)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474278
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84188 | FAR1 | fatty acyl-CoA reductase 1 | NM_032228.6 | 99.9% | .7% | 5delT |
| 2 | human | 84188 | FAR1 | fatty acyl-CoA reductase 1 | XM_011520400.2 | 99.3% | .7% | 5delT;886_894delAGGTATATG |
| 3 | mouse | 67420 | Far1 | fatty acyl CoA reductase 1 | NM_027379.3 | 92.7% | .5% | (many diffs) |
| 4 | mouse | 67420 | Far1 | fatty acyl CoA reductase 1 | XM_006508127.3 | 92.7% | .5% | (many diffs) |
| 5 | mouse | 67420 | Far1 | fatty acyl CoA reductase 1 | XM_006508128.3 | 92.7% | .5% | (many diffs) |
| 6 | mouse | 67420 | Far1 | fatty acyl CoA reductase 1 | XM_006508129.3 | 92.7% | .5% | (many diffs) |
| 7 | mouse | 67420 | Far1 | fatty acyl CoA reductase 1 | XM_006508130.3 | 92.7% | .5% | (many diffs) |
| 8 | mouse | 67420 | Far1 | fatty acyl CoA reductase 1 | XM_006508131.3 | 92.7% | .5% | (many diffs) |
| 9 | mouse | 67420 | Far1 | fatty acyl CoA reductase 1 | NM_001285831.1 | 88% | .5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 129
- ORF length:
- 63
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt tcaatcccag aatactatga aggcaagaac gtcctcctca caggagctac 121 cggttttcta gggaaggtgc ttctggaaaa gttgctgagg tcttgtccta aggtgaattc 181 agtatatgtt ttggtgaggc agaaagctgg acagacacca caagagcgag tggaagaagt 241 ccttagtggc aagctttttg acagattgag agatgaaaat ccagatttta gagagaaaat 301 tatagcaatc aacagcgaac tcacccaacc taaactggct ctcagtgaag aagataaaga 361 ggtgatcata gattctacca atattatatt ccactgtgca gctacagtaa ggtttaatga 421 aaatttaaga gatgctgttc agttaaatgt gattgcaacg cgacagctta ttctccttgc 481 acaacaaatg aagaatctgg aagtgttcat gcatgtatca acagcatatg cctactgtaa 541 tcgcaagcat attgatgaag tagtctatcc accacctgtg gatcccaaga agctgattga 601 ttctttagag tggatggatg atggcctagt aaatgatatc acgccaaaat tgataggaga 661 cagacctaat acatacatat acacaaaagc attggcagaa tatgttgtac aacaagaagg 721 agcaaaacta aatgtggcaa ttgtaaggcc atcgattgtt ggtgccagtt ggaaagaacc 781 ttttccagga tggattgata actttaatgg accaagtggt ctctttattg cggcagggaa 841 aggaattctt cgaacaatac gtgcctccaa caatgccctt gcagatcttg ttcctgtaga 901 tgtagttgtc aacatgagtc ttgcggcagc ctggtattcc ggagttaata gaccaagaaa 961 catcatggtg tataattgta caacaggcag cactaatcct ttccactggg gtgaagttga 1021 gtaccatgta atttccactt tcaagaggaa tcctctcgaa caggccttca gacggcccaa 1081 tgtaaatcta acctccaaTC ATCTTTTATA TCATTACTGG ATTGCTGTAA GCCATAAGGC 1141 CCCAGCATTC CTGTATGATA TCTACCTCAG GATGACTGGA AGAAGCCCAA GGATGATGAA 1201 AACAATAACT CGTCTTCACA AAGCTATGGT GTTTCTTGAA TATTTCACAA GTAATTCTTG 1261 GGTTTGGAAT ACTGAGAATG TCAATATGTT AATGAATCAA CTAAACCCTG AAGATAAAAA 1321 GACCTTCAAT ATTGATGTAC GGCAGTTACA TTGGGCAGAA TATATAGAGA ACTACTGCTT 1381 GGGAACTAAG AAGTACGTAT TGAATGAAGA AATGTCTGGC CTCCCTGCAG CCAGAAAACA 1441 TCTGAACAAG TTGCGGAATA TACGTTATGG TTTTAATACT ATCCTTGTGA TCCTCATCTG 1501 GCGCATTTTT ATTGCAAGAT CACAAATGGC AAGAAATATC TGGTACTTTG TGGTTAGTCT 1561 GTGTTACAAG TTTTTGTCAT ACTTCCGAGC ATCCAGCACT ATGAGATACT GCCCAACTTT 1621 CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC 1681 TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 1741 TTGTGGAAAG GACGAGGGCA AAGCCCTTCA TGATTTGATA CGCGTTAAGT Cgacaatcaa 1801 cctctggatt acaaaatttg tgaaagatt