Construct: ORF TRCN0000474300
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008091.1_s317c1
- Derived from:
- ccsbBroadEn_02403
- DNA Barcode:
- CCTGTTGCCCGGTGAGCCCGTGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CNPY2 (10330)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474300
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10330 | CNPY2 | canopy FGF signaling regula... | NM_014255.7 | 100% | 100% | |
| 2 | human | 10330 | CNPY2 | canopy FGF signaling regula... | NM_001190991.2 | 41.5% | 38.9% | (many diffs) |
| 3 | mouse | 56530 | Cnpy2 | canopy FGF signaling regula... | NM_019953.1 | 91.5% | 97.2% | (many diffs) |
| 4 | mouse | 56530 | Cnpy2 | canopy FGF signaling regula... | XM_006513902.3 | 91.5% | 97.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 612
- ORF length:
- 546
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa aggctggggt tggctggccc tgcttctggg ggccctgctg ggaaccgcct 121 gggctcggag gagccaggat ctccactgtg gagcatgcag ggcTCTGGTG GATGAACTAG 181 AATGGGAAAT TGCCCAGGTG GACCCCAAGA AGACCATTCA GATGGGATCT TTCCGGATCA 241 ATCCAGATGG CAGCCAGTCA GTGGTGGAGG TGCCTTATGC CCGCTCAGAG GCCCACCTCA 301 CAGAGCTGCT GGAGGAGATA TGTGACCGGA TGAAGGAGTA TGGGGAACAG ATTGATCCTT 361 CCACCCATCG CAAGAACTAC GTACGTGTAG TGGGCCGGAA TGGAGAATCC AGTGAACTGG 421 ACCTACAAGG CATCCGAATC GACTCAGATA TTAGCGGCAC CCTCAAGTTT GCGTGTGAGA 481 GCATTGTGGA GGAATACGAG GATGAACTCA TTGAATTCTT TTCCCGAGAG GCTGACAATG 541 TTAAAGACAA ACTTTGCAGT AAGCGAACAG ATCTTTGTGA CCATGCCCTG CACATATCGC 601 ATGATGAGCT ATGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 661 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 721 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCT GTTGCCCGGT GAGCCCGTGT 781 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t