Construct: ORF TRCN0000474339
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002575.1_s317c1
- Derived from:
- ccsbBroadEn_12221
- DNA Barcode:
- GCTCGCCTCTTGTAGCTCAGTAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASIC4 (55515)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474339
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55515 | ASIC4 | acid sensing ion channel su... | NM_182847.2 | 64% | 63.8% | (many diffs) |
| 2 | human | 55515 | ASIC4 | acid sensing ion channel su... | NM_018674.5 | 62.2% | 62% | (many diffs) |
| 3 | mouse | 241118 | Asic4 | acid-sensing (proton-gated)... | NM_183022.3 | 40.1% | 42% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1317
- ORF length:
- 1251
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gagcggagcg gctggggctg cgcggcgtgg cggagcagcg ctcgctccct 121 cgctcactcg ctcgctcgca gggacacacg caggggctga cagctgtgct ggtgctgata 181 agggaagcca caaggagacg atcgaggaga gagacaagcg gcagcagagg cagcagcggc 241 agaggcagca ccagggctgc ggagctgctg ggagtgggag tgactccccc acctcgggcc 301 cccaccctgt ccctgtcctc ttcccgcttg ccctgagttt agaagagcag ccgctgccac 361 cactgccact cgggagggca ccagggctgc tggctaggga gggacagggc agggaggctc 421 tggccagtcc cagcagccgg ggacagatgc cgatcgagat tgtgtgcaaa atcaaatttg 481 ctgaggagga tgcgaaaccc aaggagaagg aggcagggga tgagcagagc ctcctcgggg 541 ctgttgcccc tggagcagcc ccccgagacc tggccacctt tgccagcacc agcaccctgc 601 atggactggg ccgggcctgt ggcccaggcc cccacggact gcgcagaacc ctgtgggcac 661 tggccctact cacctcgctg gctgccttcc tgtaccaggc ggctggcctg gcccggggct 721 acctgacccg gcctcacctg gtggcaatgg accccgctgc cccagcccca gtggcgggct 781 tcccggctgt caccctctgc aatatcaacc gcttccggca ttcggcactc agcgatgccg 841 acatcttcca cctggccaat ctgacagggc tgccccccaa agaccgggat gggcaccgtg 901 cggctggcct gcgctaccca gagcctgaca tggtagacat ccTCAACCGC ACTGGCCACC 961 AGCTCGCCGA CATGCTTAAG AGCTGCAACT TCAGTGGGCA TCACTGCTCC GCCAGCAACT 1021 TCTCTGTGGT CTATACTCGC TATGGGAAGT GTTACACCTT CAACGCGGAC CCGCGGAGCT 1081 CGCTGCCCAG CCGGGCAGGG GGCATGGGCA GTGGCCTGGA GATCATGCTG GACATCCAGC 1141 AGGAGGAGTA CCTGCCCATC TGGAGGGAGA CAAATGAGAC GTCGTTTGAG GCAGGTATTC 1201 GGGTGCAGAT CCACAGCCAG GAGGAGCCGC CCTACATCCA CCAGCTGGGG TTCGGGGTGT 1261 CCCCAGGCTT CCAGACCTTT GTGTCCTGCC AGGAACAGCG GGTGAGCATC TCCTGCTACC 1321 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1381 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1441 ATATATCTTG TGGAAAGGAC GAGCTCGCCT CTTGTAGCTC AGTAAGACGC GTTAAGTCga 1501 caatcaacct ctggattaca aaatttgtga aagatt