Transcript: Human NM_182847.2

Homo sapiens acid sensing ion channel subunit family member 4 (ASIC4), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-03
Taxon:
Homo sapiens (human)
Gene:
ASIC4 (55515)
Length:
2857
CDS:
175..2118

Additional Resources:

NCBI RefSeq record:
NM_182847.2
NBCI Gene record:
ASIC4 (55515)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_182847.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000443972 CGTCGTTTGAGGCAGGTATTC pLKO_005 1289 CDS 100% 10.800 15.120 N ASIC4 n/a
2 TRCN0000439481 ACTCAGCGATGCCGACATCTT pLKO_005 936 CDS 100% 4.950 6.930 N ASIC4 n/a
3 TRCN0000155568 CCGATCGAGATTGTGTGCAAA pLKO.1 559 CDS 100% 4.950 6.930 N ASIC4 n/a
4 TRCN0000155507 CGACATGCTTAAGAGCTGCAA pLKO.1 1077 CDS 100% 2.640 2.112 N ASIC4 n/a
5 TRCN0000418989 GACAGATGGGCCTGTTCATTG pLKO_005 1832 CDS 100% 10.800 7.560 N Asic4 n/a
6 TRCN0000155199 GACACGCTATGGGAAAGAGAT pLKO.1 1629 CDS 100% 4.950 3.465 N ASIC4 n/a
7 TRCN0000155307 GCTTTGCACAAAGGTCCTTCT pLKO.1 2273 3UTR 100% 4.050 2.835 N ASIC4 n/a
8 TRCN0000155351 CCTCTGCAATATCAACCGCTT pLKO.1 903 CDS 100% 2.160 1.512 N ASIC4 n/a
9 TRCN0000416736 ATGGGAAGTGTTACACCTTTA pLKO_005 1151 CDS 100% 10.800 7.560 N Asic4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_182847.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08539 pDONR223 100% 99.8% 99.6% None 1847G>T;1856T>C n/a
2 ccsbBroad304_08539 pLX_304 0% 99.8% 99.6% V5 1847G>T;1856T>C n/a
3 TRCN0000466045 TTGGCCCTCATGACACCCTCCTCA pLX_317 16.7% 99.8% 99.6% V5 1847G>T;1856T>C n/a
4 ccsbBroadEn_12221 pDONR223 100% 64% 63.8% None (many diffs) n/a
5 ccsbBroad304_12221 pLX_304 0% 64% 63.8% V5 (many diffs) n/a
6 TRCN0000474339 GCTCGCCTCTTGTAGCTCAGTAAG pLX_317 30.2% 64% 63.8% V5 (many diffs) n/a
7 ccsbBroadEn_15899 pDONR223 0% 38.5% 34.9% None (many diffs) n/a
8 ccsbBroad304_15899 pLX_304 0% 38.5% 34.9% V5 (many diffs) n/a
9 TRCN0000479137 ATACAGAATGTATTGCAATAAACT pLX_317 47% 38.5% 34.9% V5 (many diffs) n/a
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