Construct: ORF TRCN0000474358
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015227.1_s317c1
- Derived from:
- ccsbBroadEn_06969
- DNA Barcode:
- TGTGTGTGCCGCGTCATACAACGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC18A1 (6570)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474358
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6570 | SLC18A1 | solute carrier family 18 me... | NM_001142324.2 | 99.8% | 99.5% | 10A>C;407T>C |
| 2 | human | 6570 | SLC18A1 | solute carrier family 18 me... | XM_011544626.1 | 99.8% | 99.5% | 10A>C;407T>C |
| 3 | human | 6570 | SLC18A1 | solute carrier family 18 me... | NM_001135691.2 | 93.7% | 93.5% | 10A>C;407T>C;919_1014del |
| 4 | human | 6570 | SLC18A1 | solute carrier family 18 me... | NM_003053.4 | 93.7% | 93.5% | 10A>C;407T>C;919_1014del |
| 5 | human | 6570 | SLC18A1 | solute carrier family 18 me... | XM_011544625.1 | 88.4% | 88% | (many diffs) |
| 6 | human | 6570 | SLC18A1 | solute carrier family 18 me... | NM_001142325.1 | 83.6% | 75.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1545
- ORF length:
- 1479
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ccggcccatt ctggatgctc cccagcggtt gctgaaggag gggagagcgt 121 cccggcagct ggtgctggtg gtggtattcg tcgctttgct cctggacaac atgctgttta 181 ctgtggtggt gccaattgtg cccaccttcc tatatgacat ggagttcaaa gaagtcaact 241 cttctctgca cctcggccat gccggaagtt ccccacatgc cctcgcctct cctgcctttt 301 ccaccatctt ctccttcttc aacaacaaca ccgtggctgt tgaagaaagc gtacctagtg 361 gaatagcatg gatgaatgac actgccagca ccatcccacc tccagccact gaagccatct 421 cagctcataa aaacaactgc ttgcaaggca caggtttctt ggaggaagag actacccggg 481 tcggggttct gtttgcttca aaggctgtga tgcaacttct ggtcaaccca ttcgtgggcc 541 ctctcaccaa caggattgga tatcatatcc ccatgtttgc tggctttgtt atcatgtttc 601 tctccacagt tatgtttgct ttttctggga cctatactct actctttgtg gcccgaaccc 661 ttcaaggcat tggatcttca ttttcatctg ttgcaggtct tggaatgctg gccagtgtct 721 acactgatga ccatgagaga ggacgagcca tgggaactgc tctggggggc ctggccttgg 781 ggttgctggt gggagctccc tttggaagtg taatgtacga gtttgttggg aagtctgcac 841 ccttcctcat cctggccttc ctggcactac tggatggagc actccagctt tgcatcctac 901 agccttccaa agtctctcct gagagtgcca aggggactcc cctctttatg cttctcaaag 961 acccttacat cctggtggct gcaggtctag ctttcttgcc tgccagtgtg TCCTACCTCA 1021 TTGGCACCAA CCTCTTTGGT GTGTTGGCCA ACAAGATGGG TCGGTGGCTG TGTTCCCTAA 1081 TCGGGATGCT GGTAGTAGGT ACCAGCTTGC TCTGTGTTCC TCTGGCTCAC AATATTTTTG 1141 GTCTCATTGG CCCCAATGCA GGGCTTGGCC TTGCCATAGG CATGGTGGAT TCTTCTATGA 1201 TGCCCATCAT GGGGCACCTG GTGGATCTAC GCCACACCTC GGTGTATGGG AGTGTCTACG 1261 CCATCGCTGA TGTGGCTTTT TGCATGGGCT TTGCTATAGG TCCATCCACC GGTGGTGCCA 1321 TTGTAAAGGC CATCGGTTTT CCCTGGCTCA TGGTCATCAC TGGGGTCATC AACATCGTCT 1381 ATGCTCCACT CTGCTACTAC CTGCGGAGCC CCCCGGCAAA GGAAGAGAAG CTTGCTATTC 1441 TGAGTCAGGA CTGCCCCATG GAGACCCGGA TGTATGCAAC CCAGAAGCCC ACGAAGGAAT 1501 TTCCTCTGGG GGAGGACAGT GATGAGGAGC CTGACCATGA GGAGTACCCA ACTTTCTTGT 1561 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1621 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1681 GAAAGGACGA TGTGTGTGCC GCGTCATACA ACGAACGCGT TAAGTCgaca atcaacctct 1741 ggattacaaa atttgtgaaa gatt