Construct: ORF TRCN0000474360
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001435.1_s317c1
- Derived from:
- ccsbBroadEn_06803
- DNA Barcode:
- CTCAGACGGATCAGCGACCGCTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSMC1 (5700)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474360
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5700 | PSMC1 | proteasome 26S subunit, ATP... | NM_002802.3 | 99.9% | 100% | 345C>T |
| 2 | human | 5700 | PSMC1 | proteasome 26S subunit, ATP... | NM_001330212.1 | 83.3% | 83.4% | 0_1ins219;126C>T |
| 3 | human | 5700 | PSMC1 | proteasome 26S subunit, ATP... | XM_017021470.1 | 83.3% | 83.4% | 0_1ins219;126C>T |
| 4 | mouse | 19179 | Psmc1 | protease (prosome, macropai... | NM_008947.3 | 91.7% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1386
- ORF length:
- 1320
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg tcaaagtcag agtggtggtc atggtcctgg aggtggcaag aaggatgaca 121 aggacaagaa aaagaaatat gaacctcctg taccaactag agtggggaaa aagaagaaga 181 aaacaaaggg accagatgct gccagcaaac tgccactggt gacacctcac actcagtgcc 241 ggttaaaatt actgaagtta gagagaatta aagactatct tctcatggag gaagaattca 301 ttagaaatca ggaacaaatg aaaccattag aagaaaagca agaggaggaa agatcaaaag 361 tggatgatct gagggggacc ccgatgtcag taggaacctt ggaagagatt attgatgaca 421 atcatgccat cgtgtctaca tctgtgggct cagaacacta cgtcagcatt ctttcatttg 481 tagacaagga tctgctggaa cctggctgct cggtcctgct caaccacaag gtgcatgccg 541 tgataggggt gctgatggat gacacggatc ccctggtcac agtgatgaag gtagaaaagg 601 ccccccagga gacctatgca gatattgggg ggttggacaa ccaaattcag gaaattaagg 661 aatctgtgga gcttcctctc acccatcctg aatattatga agagatgggt ataaagcctc 721 ctaagggggt cattctctat ggtccacctg gcacaggtaa aaccttgtta gccaaagcag 781 tagcaaacca aacctcagcc actttcttga gagtggttgg ctctgaactt attcagaagt 841 acctaggtga tgggcccaaa ctcgtacggg aattgTTCCG AGTTGCTGAA GAACATGCAC 901 CGTCCATCGT GTTTATTGAT GAAATTGACG CCATTGGGAC AAAAAGATAT GACTCCAATT 961 CTGGTGGTGA GAGAGAAATT CAGCGAACAA TGTTGGAACT GCTGAACCAG TTGGATGGAT 1021 TTGATTCTAG GGGAGATGTG AAAGTTATCA TGGCCACAAA CCGAATAGAA ACTTTGGATC 1081 CAGCACTTAT CAGACCAGGC CGCATTGACA GGAAGATTGA GTTCCCCCTG CCTGATGAAA 1141 AGACGAAGAA GCGCATCTTT CAGATTCACA CAAGCAGGAT GACGCTGGCT GATGATGTAA 1201 CCCTGGACGA CCTGATCATG GCTAAAGATG ACCTCTCTGG TGCTGACATC AAGGCAATCT 1261 GTACAGAAGC TGGTCTGATG GCCTTAAGAG AACGTAGAAT GAAAGTAACA AATGAAGACT 1321 TCAAAAAATC TAAAGAAAAT GTTCTTTATA AGAAACAGGA AGGCACCCCT GAGGGGCTGT 1381 ATCTCTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1441 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1501 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTCAGACGG ATCAGCGACC GCTAAACGCG 1561 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt