Construct: ORF TRCN0000474367
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000944.1_s317c1
- Derived from:
- ccsbBroadEn_10073
- DNA Barcode:
- AAGACGCACGGGCGAAAAAAGAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LHFPL3 (375612)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474367
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 375612 | LHFPL3 | LHFPL tetraspan subfamily m... | NM_199000.2 | 99.7% | 99.5% | 21T>C;52G>A |
| 2 | human | 375612 | LHFPL3 | LHFPL tetraspan subfamily m... | XM_005250327.2 | 95.8% | 95.7% | (many diffs) |
| 3 | mouse | 269629 | Lhfpl3 | lipoma HMGIC fusion partner... | NM_029990.1 | 94% | 97.4% | (many diffs) |
| 4 | mouse | 269629 | Lhfpl3 | lipoma HMGIC fusion partner... | NM_001081231.2 | 90.3% | 93.6% | (many diffs) |
| 5 | mouse | 269629 | Lhfpl3 | lipoma HMGIC fusion partner... | XM_006535717.3 | 90.3% | 93.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 777
- ORF length:
- 708
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcccggagcc gccgccgccg ccgccgccgc cgccgccgcg atgctcccga 121 ctcaggaggc tgccaagctg taccacacca actatgtgcg gaactcgcgg gccatcggcg 181 tgctgtgggc catcttcacc atctgctttg ccatcgtcaa cgtggtgtgc ttcatccagc 241 cctactggat aggcgacggc gtggacaccc cgcaagccgg ctatttcggg ctcttccact 301 actgcatcgg caacggcttc tcccgggagc tgacctgcag gggcagcttc acggacttct 361 ccacgctgcc ctcgggcgcc ttcaaagccg cctccTTCTT TATCGGCCTC TCCATGATGC 421 TCATCATTGC CTGCATCATT TGCTTTACCC TCTTCTTCTT CTGCAACACG GCCACTGTGT 481 ACAAGATATG TGCCTGGATG CAGCTCACCT CCGCTGCCTG CCTTGTGCTT GGCTGTATGA 541 TTTTCCCTGA TGGCTGGGAC TCAGATGAAG TAAAACGGAT GTGTGGAGAA AAGACAGACA 601 AGTACACTCT TGGGGCTTGC TCAGTCCGCT GGGCATACAT CCTGGCTATT ATTGGAATTT 661 TGGATGCCCT GATCCTCTCA TTTCTAGCAT TTGTGCTTGG TAATCGACAA GACAGCTTGA 721 TGGCAGAGGA ACTGAAGGCA GAAAACAAAG TTCTGCTAAG CCAATATTCT CTAGAATTGC 781 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 841 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 901 ATATATCTTG TGGAAAGGAC GAAAGACGCA CGGGCGAAAA AAGAGCACGC GTTAAGTCga 961 caatcaacct ctggattaca aaatttgtga aagatt