Construct: ORF TRCN0000474371
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014483.1_s317c1
- Derived from:
- ccsbBroadEn_14244
- DNA Barcode:
- CATACTCACATACATTTAAAGCAC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARHGEF28 (64283)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474371
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64283 | ARHGEF28 | Rho guanine nucleotide exch... | XM_017009703.1 | 47.7% | 47.4% | (many diffs) |
| 2 | human | 64283 | ARHGEF28 | Rho guanine nucleotide exch... | NM_001244364.2 | 44% | 43.8% | (many diffs) |
| 3 | human | 64283 | ARHGEF28 | Rho guanine nucleotide exch... | XM_005248568.4 | 43.3% | 43% | (many diffs) |
| 4 | human | 64283 | ARHGEF28 | Rho guanine nucleotide exch... | XM_017009702.1 | 37.6% | 37.3% | (many diffs) |
| 5 | human | 64283 | ARHGEF28 | Rho guanine nucleotide exch... | NM_001177693.1 | 35.9% | 35.7% | (many diffs) |
| 6 | human | 64283 | ARHGEF28 | Rho guanine nucleotide exch... | XM_011543547.2 | 35.5% | 35.2% | (many diffs) |
| 7 | human | 64283 | ARHGEF28 | Rho guanine nucleotide exch... | NM_001080479.2 | 35.4% | 35.2% | (many diffs) |
| 8 | human | 64283 | ARHGEF28 | Rho guanine nucleotide exch... | XM_011543546.2 | 35.4% | 35.2% | (many diffs) |
| 9 | mouse | 110596 | Arhgef28 | Rho guanine nucleotide exch... | XM_006517521.3 | 14.6% | 15.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1911
- ORF length:
- 1845
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat tgcaacagtg gatttaaaag tcaatgaata tgagaaaaac caaaaatggc 121 ttgagatcct aaataagatt gaaaacaaaa catacacgaa gctcaaaaat ggacatgtgt 181 ttaggaagca ggcactgatg agtgaagaaa ggactctgtt atatgatggc cttgtttact 241 ggaaaactgc tacaggtcgt ttcaaagata tcctagctct acttctaact gatgtgctgc 301 tctttttaca agaaaaagac cagaaataca tctttgcagc cgttgatcag aagccatcag 361 ttatttccct tcaaaagctt attgctagag aagttgctaa tgaggagaga ggaatgtttc 421 tgatcagtgc ttcatctgct ggtcctgaga tgtatgaaat tcacaccaat tccaaggagg 481 aacgcaataa ctggatgaga cggatccagc aggctgtaga aagttgtcct gaagaaaaag 541 ggggaaggac aagtgaatct gatgaagaca agaggaaagc tgaagccaga gtggccaaaa 601 ttcagcaatg tcaagaaata ctcactaacc aagaccaaca aatttgtgcg tatttggagg 661 agaagctgca tatctatgct gaacttggag aactgagcgg atttgaggac gtccatctag 721 agccccacct ccttattaaa cctgacccag gcgagcctcc ccaggcagcc tcattactgg 781 cagcagcact gaaagaagct gagagcctac aagttgcagt gaaggcctca cagatgggcg 841 ccgtgagtca atcatgtgag gacagttgtg gagactctgt cttggcggac acactcagtt 901 ctcatgatgt accaggatca ccgactgcct cattagtcac aggagggaga gaaggaagag 961 gctgttcgga tgtggatccc gggatccagg gtgtggtaac cgacttggcc gtctctgatg 1021 caggggagaa ggtggaatgt agaaattttc caggttcttc acaatcagag attatacaag 1081 ccatacagaa tttaacccgt ctcttataca gccttcaggc cgccttgacc attcaggaca 1141 gccacattga gatccacagg ctggttctcc agcagcagga gggcctgtct ctcggccact 1201 ctatcctccg aggcggcccc ttgcaggacc agaagtctcg cgacgcggac aggcagcatg 1261 aggagctggc caatgtgcac cagcttcagc accagctcca gcaggagcag cggcgctggc 1321 tgcgcaggtg tgagcagcag cagcgggcgc aggcgaccag ggagagctgg ctgcaggagc 1381 gggagcggga gtgccagtcg caggaggagc tgctgctgcg gagccggggc gagctggacc 1441 tccagcTCCA GGAGTACCAG CACAGCCTGG AGCGGCTGAG GGAGGGCCAG CGCCTGGTGG 1501 AGAGGGAGCA GGCGAGGATG CGGGCCCAGC AGAGCCTGCT GGGCCACTGG AAGCACGGCC 1561 GGCAGAGGAG CCTGCCCGCG GTGCTCCTTC CGGGTGGCCC CGAGGTAATG GAACTTAATC 1621 GATCTGAGAG TTTATGTCAT GAAAACTCAT TCTTCATCAA TGAAGCTTTA GTACAAATGT 1681 CATTTAACAC TTTCAACAAA CTGAATCCAT CAGTTATCCA TCAGGATGCC ACTTACCCTA 1741 CAACTCAATC TCATTCTGAC TTGGTGAGGA CTAGTGAACA TCAAGTAGAC CTCAAGGTGG 1801 ACCCTTCTCA GCCTTCGAAT GTCAGTCACA AACTGTGGAC AGCCGCTGGT TCCGGCCATC 1861 AGATACTTCC TTTCCATGAA AGCAGCAAGG ATCTTGTAAA AATGGTAATT ACCCAACTTT 1921 CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC 1981 TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 2041 TTGTGGAAAG GACGACATAC TCACATACAT TTAAAGCACA CGCGTTAAGT Cgacaatcaa 2101 cctctggatt acaaaatttg tgaaagatt