Construct: ORF TRCN0000474371

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014483.1_s317c1
Derived from:
ccsbBroadEn_14244
DNA Barcode:
CATACTCACATACATTTAAAGCAC
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ARHGEF28 (64283)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474371
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64283 ARHGEF28 Rho guanine nucleotide exch... XM_017009703.1 47.7% 47.4% (many diffs)
2 human 64283 ARHGEF28 Rho guanine nucleotide exch... NM_001244364.2 44% 43.8% (many diffs)
3 human 64283 ARHGEF28 Rho guanine nucleotide exch... XM_005248568.4 43.3% 43% (many diffs)
4 human 64283 ARHGEF28 Rho guanine nucleotide exch... XM_017009702.1 37.6% 37.3% (many diffs)
5 human 64283 ARHGEF28 Rho guanine nucleotide exch... NM_001177693.1 35.9% 35.7% (many diffs)
6 human 64283 ARHGEF28 Rho guanine nucleotide exch... XM_011543547.2 35.5% 35.2% (many diffs)
7 human 64283 ARHGEF28 Rho guanine nucleotide exch... NM_001080479.2 35.4% 35.2% (many diffs)
8 human 64283 ARHGEF28 Rho guanine nucleotide exch... XM_011543546.2 35.4% 35.2% (many diffs)
9 mouse 110596 Arhgef28 Rho guanine nucleotide exch... XM_006517521.3 14.6% 15.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1911
ORF length:
1845
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgat tgcaacagtg gatttaaaag tcaatgaata tgagaaaaac caaaaatggc
121ttgagatcct aaataagatt gaaaacaaaa catacacgaa gctcaaaaat ggacatgtgt
181ttaggaagca ggcactgatg agtgaagaaa ggactctgtt atatgatggc cttgtttact
241ggaaaactgc tacaggtcgt ttcaaagata tcctagctct acttctaact gatgtgctgc
301tctttttaca agaaaaagac cagaaataca tctttgcagc cgttgatcag aagccatcag
361ttatttccct tcaaaagctt attgctagag aagttgctaa tgaggagaga ggaatgtttc
421tgatcagtgc ttcatctgct ggtcctgaga tgtatgaaat tcacaccaat tccaaggagg
481aacgcaataa ctggatgaga cggatccagc aggctgtaga aagttgtcct gaagaaaaag
541ggggaaggac aagtgaatct gatgaagaca agaggaaagc tgaagccaga gtggccaaaa
601ttcagcaatg tcaagaaata ctcactaacc aagaccaaca aatttgtgcg tatttggagg
661agaagctgca tatctatgct gaacttggag aactgagcgg atttgaggac gtccatctag
721agccccacct ccttattaaa cctgacccag gcgagcctcc ccaggcagcc tcattactgg
781cagcagcact gaaagaagct gagagcctac aagttgcagt gaaggcctca cagatgggcg
841ccgtgagtca atcatgtgag gacagttgtg gagactctgt cttggcggac acactcagtt
901ctcatgatgt accaggatca ccgactgcct cattagtcac aggagggaga gaaggaagag
961gctgttcgga tgtggatccc gggatccagg gtgtggtaac cgacttggcc gtctctgatg
1021caggggagaa ggtggaatgt agaaattttc caggttcttc acaatcagag attatacaag
1081ccatacagaa tttaacccgt ctcttataca gccttcaggc cgccttgacc attcaggaca
1141gccacattga gatccacagg ctggttctcc agcagcagga gggcctgtct ctcggccact
1201ctatcctccg aggcggcccc ttgcaggacc agaagtctcg cgacgcggac aggcagcatg
1261aggagctggc caatgtgcac cagcttcagc accagctcca gcaggagcag cggcgctggc
1321tgcgcaggtg tgagcagcag cagcgggcgc aggcgaccag ggagagctgg ctgcaggagc
1381gggagcggga gtgccagtcg caggaggagc tgctgctgcg gagccggggc gagctggacc
1441tccagcTCCA GGAGTACCAG CACAGCCTGG AGCGGCTGAG GGAGGGCCAG CGCCTGGTGG
1501AGAGGGAGCA GGCGAGGATG CGGGCCCAGC AGAGCCTGCT GGGCCACTGG AAGCACGGCC
1561GGCAGAGGAG CCTGCCCGCG GTGCTCCTTC CGGGTGGCCC CGAGGTAATG GAACTTAATC
1621GATCTGAGAG TTTATGTCAT GAAAACTCAT TCTTCATCAA TGAAGCTTTA GTACAAATGT
1681CATTTAACAC TTTCAACAAA CTGAATCCAT CAGTTATCCA TCAGGATGCC ACTTACCCTA
1741CAACTCAATC TCATTCTGAC TTGGTGAGGA CTAGTGAACA TCAAGTAGAC CTCAAGGTGG
1801ACCCTTCTCA GCCTTCGAAT GTCAGTCACA AACTGTGGAC AGCCGCTGGT TCCGGCCATC
1861AGATACTTCC TTTCCATGAA AGCAGCAAGG ATCTTGTAAA AATGGTAATT ACCCAACTTT
1921CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC
1981TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC
2041TTGTGGAAAG GACGACATAC TCACATACAT TTAAAGCACA CGCGTTAAGT Cgacaatcaa
2101cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)