Transcript: Human NM_001177693.1

Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-06-07
Taxon:
Homo sapiens (human)
Gene:
ARHGEF28 (64283)
Length:
6291
CDS:
177..5294

Additional Resources:

NCBI RefSeq record:
NM_001177693.1
NBCI Gene record:
ARHGEF28 (64283)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001177693.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000256483 ATGACTAGCCCTCGGAATAAA pLKO_005 2064 CDS 100% 15.000 21.000 N ARHGEF28 n/a
2 TRCN0000256484 ACTCGTTTGGTGCGTGAATTA pLKO_005 1881 CDS 100% 13.200 18.480 N ARHGEF28 n/a
3 TRCN0000047421 CCAACAAATTTGTGCGTATTT pLKO.1 3854 CDS 100% 13.200 18.480 N LOC401198 n/a
4 TRCN0000047418 CCCGAGGTAATGGAACTTAAT pLKO.1 4818 CDS 100% 13.200 18.480 N LOC401198 n/a
5 TRCN0000256481 TGTTGGTCACTCAGCGTATTA pLKO_005 3166 CDS 100% 13.200 18.480 N ARHGEF28 n/a
6 TRCN0000256482 TCGCTCCTTCGATATCCTAAA pLKO_005 1202 CDS 100% 10.800 15.120 N ARHGEF28 n/a
7 TRCN0000110064 CCACACCATTAGACTTAGCTT pLKO.1 775 CDS 100% 3.000 4.200 N Arhgef28 n/a
8 TRCN0000047422 CCATCAGTTATCCATCAGGAT pLKO.1 4926 CDS 100% 2.640 2.112 N LOC401198 n/a
9 TRCN0000265817 GATTTGGATATCAGCTATATT pLKO_005 1329 CDS 100% 15.000 10.500 N ARHGEF28 n/a
10 TRCN0000047419 CCATCAGTTATTTCCCTTCAA pLKO.1 3573 CDS 100% 4.950 3.465 N LOC401198 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001177693.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14244 pDONR223 100% 35.9% 35.7% None (many diffs) n/a
2 ccsbBroad304_14244 pLX_304 0% 35.9% 35.7% V5 (not translated due to frame shift) (many diffs) n/a
3 TRCN0000474371 CATACTCACATACATTTAAAGCAC pLX_317 25.4% 35.9% 35.7% V5 (not translated due to frame shift) (many diffs) n/a
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