Construct: ORF TRCN0000474407
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008987.1_s317c1
- Derived from:
- ccsbBroadEn_09122
- DNA Barcode:
- AATTGTGTGCGAAAGCTCACAGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM120A (83862)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474407
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83862 | TMEM120A | transmembrane protein 120A | NM_031925.3 | 99.9% | 99.7% | 601A>G |
| 2 | human | 83862 | TMEM120A | transmembrane protein 120A | XM_024446953.1 | 83.5% | 79.2% | 563_665del;704A>G;732_829del |
| 3 | human | 83862 | TMEM120A | transmembrane protein 120A | NM_001317803.2 | 82.6% | 79% | 601A>G;916_917ins62;968_1107del |
| 4 | human | 83862 | TMEM120A | transmembrane protein 120A | NM_001363462.2 | 71.1% | 50.3% | (many diffs) |
| 5 | human | 83862 | TMEM120A | transmembrane protein 120A | NR_156695.2 | 68.8% | (many diffs) | |
| 6 | mouse | 215210 | Tmem120a | transmembrane protein 120A | NM_172541.2 | 87.6% | 94.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1095
- ORF length:
- 1029
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gcccccgccc ccgggcccgc tgggcgactg cctgcgggac tgggaggatc 121 tacagcagga cttccagaac atccaggaga cccatcggct ctaccgcctg aagctggagg 181 agctgaccaa acttcagaac aattgcacca gctccatcac gcggcagaag aagcggctcc 241 aggagctggc cctcgccctg aagaaatgca aaccctccct cccagcagag gccgaggggg 301 ccgcacagga gctggagaac cagatgaaag agcgccaagg cctcttcttt gacatggagg 361 cctatttgcc taagaagaat ggattgtacc tgagcctggt tctggggaac gtcaacgtca 421 cgctcctgag caagcaggct aagtttgcct acaaggacga gtatgagaag ttcaagctct 481 acctcaccat catcctcatc ctcatctcct tcacttgccg cttcctgctc aactccaggg 541 tgacagatgc tgccttcaac ttcctgctgg tctggtacta ctgcaccctg accatccggg 601 agagcatccT CATCAACAAC GGCTCCCGGA TCAAAGGCTG GTGGGTGTTC CATCACTACG 661 TGTCCGCCTT CCTGTCGGGA GTCATGCTGA CGTGGCCCGA CGGTCTCATG TACCAGAAAT 721 TCCGGAACCA ATTCCTCTCC TTTTCCATGT ACCAGAGCTT CGTGCAGTTT CTCCAGTACT 781 ACTACCAGAG CGGCTGCCTC TACCGCCTGC GGGCGCTGGG CGAGCGGCAC ACCATGGACC 841 TCACTGTGGA GGGCTTCCAG TCCTGGATGT GGCGGGGCCT CACCTTCCTG CTGCCTTTTC 901 TTTTCTTTGG ACACTTCTGG CAGCTTTTTA ACGCGCTGAC GTTGTTCAAC CTGGCCCAGG 961 ACCCTCAGTG CAAGGAGTGG CAGGTGCTTA TGTGCGGCTT TCCCTTCCTC CTCCTTTTCC 1021 TCGGCAATTT CTTCACCACC CTGAGGGTTG TGCACCACAA GTTTCACAGT CAGCGGCACG 1081 GGAGCAAGAA GGATTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1141 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1201 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AATTGTGTGC GAAAGCTCAC 1261 AGATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt