Construct: ORF TRCN0000474414
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014732.1_s317c1
- Derived from:
- ccsbBroadEn_04053
- DNA Barcode:
- ATGTTCAGCCGTCGTTCGTAGTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DERL1 (79139)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474414
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79139 | DERL1 | derlin 1 | NM_024295.6 | 100% | 100% | |
| 2 | human | 79139 | DERL1 | derlin 1 | NM_001134671.2 | 92% | 92% | 506_507ins60 |
| 3 | human | 79139 | DERL1 | derlin 1 | NM_001330601.1 | 60.1% | 60.1% | 0_1ins300 |
| 4 | human | 79139 | DERL1 | derlin 1 | NM_001363963.1 | 60.1% | 60.1% | 0_1ins300 |
| 5 | mouse | 67819 | Derl1 | Der1-like domain family, me... | NM_024207.4 | 92.1% | 97.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 819
- ORF length:
- 753
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggacatcgga gactggttca ggagcatccc ggcgatcacg cgctattggt 121 tcgccgccac cgtcgccgtg cccttggtcg gcaaactcgg cctcatcagc ccggcctacc 181 tcttcctctg gcccgaagcc ttcctttatc gctttcagat ttggaggcca atcactgcca 241 ccttttattt ccctgtgggt ccaggaactg gatttcttta tttggtcaat ttatatttct 301 tatatcagta ttcTACGCGA CTTGAAACAG GAGCTTTTGA TGGGAGGCCA GCAGACTATT 361 TATTCATGCT CCTCTTTAAC TGGATTTGCA TCGTGATTAC TGGCTTAGCA ATGGATATGC 421 AGTTGCTGAT GATTCCTCTG ATCATGTCAG TACTTTATGT CTGGGCCCAG CTGAACAGAG 481 ACATGATTGT ATCATTTTGG TTTGGAACAC GATTTAAGGC CTGCTATTTA CCCTGGGTTA 541 TCCTTGGATT CAACTATATC ATCGGAGGCT CGGTAATCAA TGAGCTTATT GGAAATCTGG 601 TTGGACATCT TTATTTTTTC CTAATGTTCA GATACCCAAT GGACTTGGGA GGAAGAAATT 661 TTCTATCCAC ACCTCAGTTT TTGTACCGCT GGCTGCCCAG TAGGAGAGGA GGAGTATCAG 721 GATTTGGTGT GCCCCCTGCT AGCATGAGGC GAGCTGCTGA TCAGAATGGC GGAGGCGGGA 781 GACACAACTG GGGCCAGGGC TTTCGACTTG GAGACCAGTG CCCAACTTTC TTGTACAAAG 841 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 901 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 961 ACGAATGTTC AGCCGTCGTT CGTAGTGGAC GCGTTAAGTC gacaatcaac ctctggatta 1021 caaaatttgt gaaagatt