Construct: ORF TRCN0000474443
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000036.1_s317c1
- Derived from:
- ccsbBroadEn_10070
- DNA Barcode:
- AAGCTGCCCGTACATACACTTCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C3orf62 (375341)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474443
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 375341 | C3orf62 | chromosome 3 open reading f... | NM_198562.3 | 99.8% | 99.6% | 211G>T |
| 2 | mouse | 112418 | 1700102P08Rik | RIKEN cDNA 1700102P08 gene | XM_006511628.2 | 82.7% | 75.3% | (many diffs) |
| 3 | mouse | 112418 | 1700102P08Rik | RIKEN cDNA 1700102P08 gene | NM_053216.2 | 80.4% | 75% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 867
- ORF length:
- 801
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ttacataaag acatggtcgc ttttaggaga aatgtctgaa aaactaagaa 121 gatgcagaaa ggaactgact gcagccattg accgggcctt tgaaggagtt agttattccc 181 aggagtgcac aggccagcag aggctggaac tgagcgccgc gccgctctcc ttctcgctgc 241 ccgtgcacag gctcctctgc agaagacatc ctctgtcagc ctgctcttct gctgctcctt 301 ttgctgctgt cccatgtgct cctgagaatg agaaccctgc ctttgcaaca aaccatgccc 361 cggtaaatgc aaaaccacat gctctgtgcc ccgagagaaa acctctaacc agcaaggaaa 421 atgtattgat gcattcctcc attttggcac ctgaaagaga gtcttggaga actgcaggag 481 aggggGAAAA CTGGAGAAAA GAAAATTTAA GGAAAGATAT GGAGAGAGAT TTGAAGGCTG 541 ACTCAAACAT GCCACTCAAC AATTCTAGCC AAGAGGTCAC AAAGGATCTG CTTGATATGA 601 TTGACCATAC AAGCATCCGA ACTATTGAAG AATTGGCTGG AAAAATAGAA TTTGAAAACG 661 AATTGAACCA CATGTGTGGT CATTGCCAAG ATTCACCCTT CAAAGAGGAA GCCTGGGCCC 721 TGCTCATGGA CAAGAGCCCT CAGAAGGCCA CAGATGCTGA CCCTGGCAGC CTCAAACAGG 781 CTTTTGATGA TCATAATATT GTTGAGACTG TTCTGGACTT GGAAGAGGAC TACAATGTGA 841 TGACGTCTTT TAAATACCAA ATTGAGTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 901 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 961 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAAGCTGCC 1021 CGTACATACA CTTCCTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1081 aagatt