Construct: ORF TRCN0000474445
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001799.1_s317c1
- Derived from:
- ccsbBroadEn_03469
- DNA Barcode:
- TTCGACGTAGATCTATGGGCGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANKRD49 (54851)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474445
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54851 | ANKRD49 | ankyrin repeat domain 49 | NM_017704.3 | 100% | 100% | |
| 2 | human | 54851 | ANKRD49 | ankyrin repeat domain 49 | XM_017017941.1 | 100% | 100% | |
| 3 | mouse | 56503 | Ankrd49 | ankyrin repeat domain 49 | NM_001348260.1 | 88.2% | 87.4% | (many diffs) |
| 4 | mouse | 56503 | Ankrd49 | ankyrin repeat domain 49 | NM_019683.4 | 88.2% | 87.4% | (many diffs) |
| 5 | mouse | 56503 | Ankrd49 | ankyrin repeat domain 49 | XM_006510503.2 | 88.2% | 87.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 783
- ORF length:
- 717
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aaaagaaaaa ggaaatgatg atggaatacc agaccaagag aattccttgg 121 atttttctga acactttaac caacttgaat tgttggaaac acatggacac cttattccta 181 ctggtactca aagtctttgg gtaggcaatt ctgatgaaga tgaggagcaa gatgacaaaa 241 atgaagagtg gtatcgattg caagaaaaaa aaatggaaaa agacccaagc agattgcttc 301 tttgggctgc tgaaaaaaat cggcttacca cagtgcggag actcctttct gaaaaggcca 361 ctcacgtgaa cactagggat gaagatgagt atacccctct tcatcgagca gccTACAGTG 421 GACACTTAGA TATTGTTCAG GAGCTCATTG CACAGGGGGC CGATGTTCAT GCAGTGACTG 481 TGGATGGCTG GACGCCCCTG CACAGTGCTT GTAAGTGGAA TAATACCAGA GTGGCTTCTT 541 TCTTACTGCA GCATGATGCA GATATCAATG CCCAAACAAA AGGCCTCTTG ACCCCCTTGC 601 ATCTTGCTGC TGGGAACAGA GACAGCAAGG ATACCCTAGA ACTCCTCCTG ATGAACCGTT 661 ACGTCAAACC AGGGCTGAAA AACAACTTGG AAGAAACTGC ATTTGATATT GCCAGGAGGA 721 CAAGTATCTA TCACTACCTC TTTGAAATTG TGGAAGGCTG TACAAATTCT TCACCTCAGT 781 CTTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 841 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 901 GGCTTTATAT ATCTTGTGGA AAGGACGATT CGACGTAGAT CTATGGGCGT GCACGCGTTA 961 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt