Construct: ORF TRCN0000474455
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003262.1_s317c1
- Derived from:
- ccsbBroadEn_13969
- DNA Barcode:
- ACAACGAGCAACAAACGTTGGTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TIA1 (7072)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474455
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NM_001351518.1 | 99.6% | 99% | 16C>G;623G>C |
| 2 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NM_001351516.2 | 82.1% | 73.8% | (many diffs) |
| 3 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NM_001351519.2 | 78.8% | 73.8% | (many diffs) |
| 4 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NM_001351521.2 | 73.6% | 68.2% | (many diffs) |
| 5 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NM_001351522.2 | 65.9% | 62.1% | (many diffs) |
| 6 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | XR_002959333.1 | 62.2% | (many diffs) | |
| 7 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NM_001351508.2 | 54.4% | 49.4% | (many diffs) |
| 8 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NM_022173.4 | 54.2% | 49.3% | (many diffs) |
| 9 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NM_001351510.2 | 51.5% | 46.4% | (many diffs) |
| 10 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NM_022037.4 | 51.4% | 46.3% | (many diffs) |
| 11 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NM_001351509.2 | 51.1% | 46% | (many diffs) |
| 12 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NM_001351520.2 | 47.3% | 51.1% | (many diffs) |
| 13 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NM_001351511.1 | 44.7% | 40.2% | (many diffs) |
| 14 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NM_001351523.2 | 43.4% | 38.3% | (many diffs) |
| 15 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NM_001351513.1 | 41.9% | 37.2% | (many diffs) |
| 16 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NM_001351512.1 | 41.7% | 37% | (many diffs) |
| 17 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | XM_005264528.5 | 37.5% | 33.1% | (many diffs) |
| 18 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NM_001351514.2 | 34.6% | 30.1% | (many diffs) |
| 19 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NM_001351515.2 | 27.9% | 23.7% | (many diffs) |
| 20 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | XM_005264531.2 | 27.9% | 23.7% | (many diffs) |
| 21 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | XM_017004795.1 | 27.9% | 23.7% | (many diffs) |
| 22 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NM_001351517.2 | 18.1% | 14.2% | (many diffs) |
| 23 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | XM_024453067.1 | 18.1% | 14.2% | (many diffs) |
| 24 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NM_001351524.2 | 18.1% | 14.1% | (many diffs) |
| 25 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NM_001351525.2 | 18.1% | 14.1% | (many diffs) |
| 26 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | XM_017004796.1 | 18.1% | 14.1% | (many diffs) |
| 27 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | XM_017004800.1 | 18.1% | 14.1% | (many diffs) |
| 28 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | XM_024453063.1 | 18.1% | 14.1% | (many diffs) |
| 29 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | XM_024453064.1 | 18.1% | 14.1% | (many diffs) |
| 30 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | XM_024453065.1 | 18.1% | 14.1% | (many diffs) |
| 31 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | XM_024453066.1 | 18.1% | 14.1% | (many diffs) |
| 32 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NR_147218.1 | 13.5% | (many diffs) | |
| 33 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NR_147217.1 | 13.5% | (many diffs) | |
| 34 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NR_147231.2 | 13.4% | (many diffs) | |
| 35 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NR_147228.2 | 13.4% | (many diffs) | |
| 36 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NR_147219.2 | 13.2% | (many diffs) | |
| 37 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NR_147216.1 | 13.2% | (many diffs) | |
| 38 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NR_147225.2 | 13% | (many diffs) | |
| 39 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NR_147223.2 | 12.9% | (many diffs) | |
| 40 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NR_147227.2 | 12.9% | (many diffs) | |
| 41 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NR_147230.2 | 12.9% | (many diffs) | |
| 42 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NR_147224.2 | 12.7% | (many diffs) | |
| 43 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NR_147226.2 | 12.7% | (many diffs) | |
| 44 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NR_147220.2 | 12.6% | (many diffs) | |
| 45 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NR_147229.2 | 12.5% | (many diffs) | |
| 46 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NR_147221.2 | 12.5% | (many diffs) | |
| 47 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NR_147222.2 | 12.4% | (many diffs) | |
| 48 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | NR_147232.2 | 12% | (many diffs) | |
| 49 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | XR_002959332.1 | 11.5% | (many diffs) | |
| 50 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | XR_002959330.1 | 10.9% | (many diffs) | |
| 51 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | XR_002959331.1 | 7% | (many diffs) | |
| 52 | human | 7072 | TIA1 | TIA1 cytotoxic granule asso... | XR_002959329.1 | 7% | (many diffs) | |
| 53 | mouse | 21841 | Tia1 | cytotoxic granule-associate... | XM_006505891.2 | 65.9% | 64.7% | (many diffs) |
| 54 | mouse | 21841 | Tia1 | cytotoxic granule-associate... | XM_017321512.1 | 63.3% | 62.3% | (many diffs) |
| 55 | mouse | 21841 | Tia1 | cytotoxic granule-associate... | NM_001164079.1 | 62.2% | 60.7% | (many diffs) |
| 56 | mouse | 21841 | Tia1 | cytotoxic granule-associate... | NM_011585.4 | 50.6% | 49.8% | (many diffs) |
| 57 | mouse | 21841 | Tia1 | cytotoxic granule-associate... | XM_006505889.2 | 47.7% | 46.8% | (many diffs) |
| 58 | mouse | 21841 | Tia1 | cytotoxic granule-associate... | NM_001164078.1 | 47.5% | 46.5% | (many diffs) |
| 59 | mouse | 21841 | Tia1 | cytotoxic granule-associate... | XM_011241285.2 | 26.1% | 24% | (many diffs) |
| 60 | mouse | 21841 | Tia1 | cytotoxic granule-associate... | XM_006505893.3 | 17.2% | 14.3% | (many diffs) |
| 61 | mouse | 21841 | Tia1 | cytotoxic granule-associate... | XM_011241286.2 | 17.2% | 14.3% | (many diffs) |
| 62 | mouse | 21841 | Tia1 | cytotoxic granule-associate... | XM_017321513.1 | 17.2% | 14.3% | (many diffs) |
| 63 | mouse | 21841 | Tia1 | cytotoxic granule-associate... | XM_017321514.1 | 17.2% | 14.3% | (many diffs) |
| 64 | mouse | 21841 | Tia1 | cytotoxic granule-associate... | XR_377438.3 | 14.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 708
- ORF length:
- 642
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggacgagatg gccaagactc tatacgtcgg taacctttcc agagatgtga 121 cagaagctct aattctgcaa ctctttagcc agattggacc ttgtaaaaac tgcaaaatga 181 ttatggatac agctggaaat gatccctatt gttttgtgga gtttcatgag catcgtcatg 241 cagcTGCAGC ATTAGCTGCT ATGAATGGAC GGAAGATAAT GGGTAAGGAA GTCAAAGTGA 301 ATTGGGCAAC AACCCCTAGC AGTCAAAAGA AAGATACAAG CAGTAGTACC GTTGTCAGCA 361 CACAGCGTTC ACAAGATCAT TTCCATGTCT TTGTTGGTGA TCTCAGCCCA GAAATTACAA 421 CTGAAGATAT AAAAGCTGCT TTTGCACCAT TTGGAAGAAT ATCAGATGCC CGAGTGGTAA 481 AAGACATGGC AACAGGAAAG TCTAAGGGAT ATGGCTTTGT CTCCTTTTTC AACAAATGGG 541 ATGCTGAAAA CGCCATTCAA CAGATGGGTG GCCAGTGGCT TGGTGGAAGA CAAATCAGAA 601 CTAACTGGGC AACCCGAAAG CCTCCCGCTC CAAAGAGTAC ATATGAGTGT AGGTGTATTG 661 GAGAAGAAAA GGAAATGTGG AATTTTGCAG AAAAATACGC TAGATTTTAC CCAACTTTCT 721 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 781 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 841 GTGGAAAGGA CGAACAACGA GCAACAAACG TTGGTTGACG CGTTAAGTCg acaatcaacc 901 tctggattac aaaatttgtg aaagatt