Construct: ORF TRCN0000474468
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009647.1_s317c1
- Derived from:
- ccsbBroadEn_10719
- DNA Barcode:
- TCCCTCAATTGAGACCGAATCTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CD48 (962)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474468
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 962 | CD48 | CD48 molecule | XM_005245625.1 | 81.5% | 77.1% | (many diffs) |
| 2 | human | 962 | CD48 | CD48 molecule | NM_001256030.1 | 62.9% | 58.7% | (many diffs) |
| 3 | human | 962 | CD48 | CD48 molecule | NM_001778.4 | 62.2% | 55.1% | (many diffs) |
| 4 | human | 962 | CD48 | CD48 molecule | XM_011510171.2 | 49.3% | 42% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 573
- ORF length:
- 507
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg ctccagaggt tgggattcgt gtctggctct ggaattgcta ctgctgcctc 121 tgtcactcct ggtgaccagc attcaaggtc acttggtaca tatgaccgtg gtctccggca 181 gcaacgtgac tctgaacatc tctgagagcc tgccTGAGAA CTACAAACAA CTAACCTGGT 241 TTTATACTTT CGACCAGAAG ATTGTAGAAT GGGATTCCAG AAAATCTAAG TACTTTGAAT 301 CCAAATTTAA AGGCAGGGTC AGACTTGATC CTCAGAGTGG CGCACTGTAC ATCTCTAAGG 361 TCCAGAAAGA GGACAACAGC ACCTACATCA TGAGGGTGTT GAAAAAGACT GGGAATGAGC 421 AAGAATGGAA GATCAAGCTG CAAGTGCTTG GTGAGTCAGG GGAGCCAAAG AGCAAATCCC 481 CACTCCAGTG GCCCCAGATG GACCACTGTA GGGCTTCCTG GGAGGCATGG GGCACTCTCG 541 GGGAGGAGGA GAGAAAAACC TCAGGACAAG TTTGCCCAAC TTTCTTGTAC AAAGTGGTTG 601 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 661 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 721 CCTCAATTGA GACCGAATCT GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 781 ttgtgaaaga tt