Construct: ORF TRCN0000474472
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014644.1_s317c1
- Derived from:
- ccsbBroadEn_07444
- DNA Barcode:
- AAACTGTGATTACTCGCAATGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEC22B (9554)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474472
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9554 | SEC22B | SEC22 homolog B, vesicle tr... | NM_004892.6 | 99.8% | 100% | 96A>G |
| 2 | human | 9554 | SEC22B | SEC22 homolog B, vesicle tr... | XM_017002856.2 | 81.9% | 76.1% | (many diffs) |
| 3 | human | 100996517 | SEC22B3P | SEC22 homolog B3, pseudogene | NR_158170.1 | 8.6% | (many diffs) | |
| 4 | human | 100996716 | SEC22B2P | SEC22 homolog B2, pseudogene | NR_158171.1 | 8.6% | (many diffs) | |
| 5 | human | 102724364 | SEC22B4P | SEC22 homolog B4, pseudogene | XR_922650.2 | 7.9% | (many diffs) | |
| 6 | mouse | 20333 | Sec22b | SEC22 homolog B, vesicle tr... | NM_011342.4 | 93.1% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 711
- ORF length:
- 645
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gttgctaaca atgatcgccc gagtggcgga cgggctcccg ctggccgcct 121 cgatgcagga ggacgaacag tctggccggg accttcaaca gtatcagagt caggctaagc 181 aactctttcg aaagttgaat gaacagtccc ctaccagatg taccttggaa gcaggagcca 241 tgacttttca ctacattatt gagcaggggg tgtgttattt ggttttatgt gaagctgcct 301 tccctaagaa gttggctttt gcctacctag aagatttgca ctcagaattt gatgaacagc 361 atGGAAAGAA GGTGCCCACT GTGTCCCGAC CCTATTCCTT TATTGAATTT GATACTTTCA 421 TTCAGAAAAC CAAGAAGCTC TACATTGACA GTCGTGCTCG AAGAAATCTA GGCTCCATCA 481 ACACTGAATT GCAAGATGTG CAGAGGATCA TGGTGGCCAA TATTGAAGAA GTGTTACAAC 541 GAGGAGAAGC ACTCTCAGCA TTGGATTCAA AGGCTAACAA TTTGTCCAGT CTGTCCAAGA 601 AATACCGCCA GGATGCGAAG TACTTGAACA TGCGTTCCAC TTATGCCAAA CTTGCAGCAG 661 TAGCTGTATT TTTCATCATG TTAATAGTGT ATGTCCGATT CTGGTGGCTG TGCCCAACTT 721 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 781 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 841 CTTGTGGAAA GGACGAAAAC TGTGATTACT CGCAATGAAC ACGCGTTAAG TCgacaatca 901 acctctggat tacaaaattt gtgaaagatt