Construct: ORF TRCN0000474472

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014644.1_s317c1
Derived from:
ccsbBroadEn_07444
DNA Barcode:
AAACTGTGATTACTCGCAATGAAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SEC22B (9554)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474472
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9554 SEC22B SEC22 homolog B, vesicle tr... NM_004892.6 99.8% 100% 96A>G
2 human 9554 SEC22B SEC22 homolog B, vesicle tr... XM_017002856.2 81.9% 76.1% (many diffs)
3 human 100996517 SEC22B3P SEC22 homolog B3, pseudogene NR_158170.1 8.6% (many diffs)
4 human 100996716 SEC22B2P SEC22 homolog B2, pseudogene NR_158171.1 8.6% (many diffs)
5 human 102724364 SEC22B4P SEC22 homolog B4, pseudogene XR_922650.2 7.9% (many diffs)
6 mouse 20333 Sec22b SEC22 homolog B, vesicle tr... NM_011342.4 93.1% 100% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
711
ORF length:
645
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt gttgctaaca atgatcgccc gagtggcgga cgggctcccg ctggccgcct
121cgatgcagga ggacgaacag tctggccggg accttcaaca gtatcagagt caggctaagc
181aactctttcg aaagttgaat gaacagtccc ctaccagatg taccttggaa gcaggagcca
241tgacttttca ctacattatt gagcaggggg tgtgttattt ggttttatgt gaagctgcct
301tccctaagaa gttggctttt gcctacctag aagatttgca ctcagaattt gatgaacagc
361atGGAAAGAA GGTGCCCACT GTGTCCCGAC CCTATTCCTT TATTGAATTT GATACTTTCA
421TTCAGAAAAC CAAGAAGCTC TACATTGACA GTCGTGCTCG AAGAAATCTA GGCTCCATCA
481ACACTGAATT GCAAGATGTG CAGAGGATCA TGGTGGCCAA TATTGAAGAA GTGTTACAAC
541GAGGAGAAGC ACTCTCAGCA TTGGATTCAA AGGCTAACAA TTTGTCCAGT CTGTCCAAGA
601AATACCGCCA GGATGCGAAG TACTTGAACA TGCGTTCCAC TTATGCCAAA CTTGCAGCAG
661TAGCTGTATT TTTCATCATG TTAATAGTGT ATGTCCGATT CTGGTGGCTG TGCCCAACTT
721TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
781CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
841CTTGTGGAAA GGACGAAAAC TGTGATTACT CGCAATGAAC ACGCGTTAAG TCgacaatca
901acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)