Construct: ORF TRCN0000474484
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012392.1_s317c1
- Derived from:
- ccsbBroadEn_09921
- DNA Barcode:
- CAAGATGCAGAGTCATAGACATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC96 (257236)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474484
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 257236 | CCDC96 | coiled-coil domain containi... | NM_153376.3 | 99.8% | 99.8% | 66C>T;286G>A;309T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1734
- ORF length:
- 1665
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacgtcagc tctgagcaca ctaaggaccc cggcggggaa ggcggagacg 121 gggaaagcct ggctgcgcgg ccgtccaaga tcaaggccag ctctggccct ccgacctctc 181 ccgaacccgg ggagctggag tcggagccgg aggaggagga ggaggagcaa gcggcttcgc 241 agggaggcac cgccgcggac gagcaggccg aggccccgaa agggctaacg gcagccgagg 301 ctgcgggcga ggaggggcct ggagagccgg ggaggccggc cgagccccag cccaagcccg 361 aagagccggc cgaggtcggg gctgaggagc ccgcccagcc ggagcccgga gccgggcccg 421 aggaactaga ggcggaggcg ggggcggagg aactggagca ggcggcagag gggaaggaag 481 tcaggttcca ggcctctctg ccgctgacca ggatcgatga ggaagaggct gctgcagccc 541 cggaggccga gacagagcgg gtggaggggg aggaagagga caaggaggag acgcagagag 601 acggcgcgga gagcaaggag agggatgggg aaggacgccc ggccaaaagc caggaagagg 661 ggaagcgcct ctatggaagg gacgagtttg aggacctcga gtggtccgag gaggtccaga 721 agctgcagga gcagcagctg cgcagcgacc tcctggacca gtaccgttcc ctgctggtgg 781 agcggaaccg ctcccagcgc tacaacctat acctgcagca caagatcttc gaggcgctgc 841 gcagaaagaa gggcctggag gccgctgagg tggctgaccg gggcgcagag gccgaggccc 901 ccgagaaaga gcaagcgtac ctgcgccatc tgggcatgct ggaggagctg aagaagcagc 961 aggcagacga cctgcagtgg taccaccagg agctgggcca gttgaagcgg cagtgccagg 1021 agaagctcac cagggtggag aaggagtggc gacgcttcca ggcactcaag aagcaggtgg 1081 tgatgcaggc catgggcagc tgtcggatga ggggcgggcg ccaggctgct ctgcgagagg 1141 tggagcagat ccaggcgttg gaggataaaa aggagaagga gatgagcgcc gtgcggctgg 1201 agaacattca gctgaagcag agcctggtgc attttgaaac caggatgagg acccaggagg 1261 acctgaccca gggtctgctt cttattgact ttgaacagct gaagattgag aaccaaacct 1321 tcaatgagaa aattgaggaa cgaaatgagg aacttttaaa acttcgcagc aaggtgacca 1381 acagtgtgca agtaataacc cacgtgaagg aaaagctgca cttcatggac atggagaacg 1441 cgtgcaaaaa gacacagctg gcagaaatcg aggcccaggc cgccctagga agagacatcc 1501 tgaccaagac gaagcaagcc cgagaggggc tgcggacgga caacatcaga ctgaatcaga 1561 agtgcggccT TCTAGGCAAG GACTCACTTC TTCGGGACTT GGAAGAAAAG GTGGACAAGA 1621 CCGAACTGCT TCACCGGCGC CTGGAATCCC TGAAGCGCCA CCACGCTAGC CTCACTTTGT 1681 CCTGCAGAGG CGTGAGGCAG AAGATCAGGG AGGCCAAAGC CTTTCTCCCT TCTTTGCCAA 1741 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1801 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1861 TATCTTGTGG AAAGGACGAC AAGATGCAGA GTCATAGACA TTAACGCGTT AAGTCgacaa 1921 tcaacctctg gattacaaaa tttgtgaaag att