Construct: ORF TRCN0000474485
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003575.1_s317c1
- Derived from:
- ccsbBroadEn_10338
- DNA Barcode:
- TGCCTTCTGTTCTGAAGACACACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HMGN2P46 (283651)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474485
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3151 | HMGN2 | high mobility group nucleos... | NM_005517.4 | 54.6% | 9.4% | (many diffs) |
| 2 | human | 283651 | HMGN2P46 | high mobility group nucleos... | NR_022014.1 | 23.3% | 1_715del;1166_1927del | |
| 3 | mouse | 627375 | Gm6750 | predicted gene 6750 | XR_873085.1 | 59.4% | (many diffs) | |
| 4 | mouse | 15331 | Hmgn2 | high mobility group nucleos... | NM_016957.3 | 52.4% | 9.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 519
- ORF length:
- 450
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcatttactt ctctGGCTAC AAAAAAGAAT AGAAATGCAC AAAGCAGAGC 121 AGTGTGAAGA AGAAGAGGCG ATGACCCCTA GACCGACCAA AGCCCGTGCT CCACTGCCCA 181 GTGCCTATGT CCCACCACTG TCGCTGCCAC CCTGCCCGAG AGAAAGGCTG AAGGGGATGC 241 TAAAGGAGAT AAAACCAAGG TTAAGTAGGA ACTGCAGAGA AGATCCACAA GGTTGTCTGC 301 TAAACCTGCT CCTCCAAAGC CACAGCCGAA GCCCAGAAAG GCCTCTGCAA AGAAGAGAGA 361 GAAGGTACCT ACAGAGAAGA AGGGAAAAGC TGATGCTGGC AAGGAGGGGA ATAACCCTGC 421 AGAAAATGGA GATGCCAAAA CAGACTAGGC ACAGAAAGCT AAAGGTGCTG GAGATGCCAA 481 GTGAAGTGTG TGCATTTTTG ATAACTGTGT ACTTCTGGTT GCCAACTTTC TTGTACAAAG 541 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 601 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 661 ACGATGCCTT CTGTTCTGAA GACACACAAC GCGTTAAGTC gacaatcaac ctctggatta 721 caaaatttgt gaaagatt