Construct: ORF TRCN0000474495
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001007.1_s317c1
- Derived from:
- ccsbBroadEn_03680
- DNA Barcode:
- CCAATCTAGTGATAACCTAGCATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZC4H2 (55906)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474495
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55906 | ZC4H2 | zinc finger C4H2-type conta... | NM_018684.4 | 100% | 100% | |
| 2 | human | 55906 | ZC4H2 | zinc finger C4H2-type conta... | NM_001178032.3 | 89.7% | 89.7% | 0_1ins69 |
| 3 | human | 55906 | ZC4H2 | zinc finger C4H2-type conta... | NM_001243804.2 | 89.7% | 89.7% | 0_1ins69 |
| 4 | human | 55906 | ZC4H2 | zinc finger C4H2-type conta... | NM_001178033.2 | 73.6% | 57.5% | 398_399ins163;510_528del |
| 5 | human | 55906 | ZC4H2 | zinc finger C4H2-type conta... | NR_045044.2 | 22.5% | 1_73del;124_378del;1001_2979del | |
| 6 | mouse | 245522 | Zc4h2 | zinc finger, C4H2 domain co... | NM_001003916.2 | 90.3% | 100% | (many diffs) |
| 7 | mouse | 245522 | Zc4h2 | zinc finger, C4H2 domain co... | XM_011247587.2 | 89.1% | 98.6% | (many diffs) |
| 8 | mouse | 245522 | Zc4h2 | zinc finger, C4H2 domain co... | NM_001289697.1 | 87.6% | 96.8% | (many diffs) |
| 9 | mouse | 245522 | Zc4h2 | zinc finger, C4H2 domain co... | NM_001289696.1 | 71% | 60.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 738
- ORF length:
- 672
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agatgagcaa gaaatcatgt gcaaattgga aagcattaaa gagatcagga 121 acaagaccct gcagatggag aagatcaagg ctcgtttgaa ggctgagttt gaggcacttg 181 agtcagagga aaggcacctg aaggaataca agcaggagat ggaccttctg ctacaggaga 241 agatggccca tgtggaggaa ctccgactga tccacgctga catcaatgtg atggaaaaca 301 ctatcaaaca atctgagaat gacctaaaca agctgctaga gtctacaagg aggcTGCATG 361 ATGAGTATAA GCCACTGAAA GAACATGTGG ATGCCCTGCG CATGACTCTG GGCCTGCAGA 421 GGCTCCCTGA CTTGTGTGAA GAAGAGGAGA AGCTTTCCTT GGATTACTTT GAGAAGCAGA 481 AAGCAGAATG GCAGACAGAA CCTCAGGAGC CCCCCATCCC TGAGTCCCTG GCCGCTGCAG 541 CCGCTGCCGC CCAACAGCTC CAAGTGGCTA GGAAGCAGGA TACTCGGCAG ACGGCCACCT 601 TCAGGCAGCA GCCCCCACCT ATGAAGGCCT GCTTGTCATG TCACCAGCAA ATTCACCGGA 661 ATGCACCTAT ATGCCCTCTT TGCAAGGCCA AGAGTCGGTC CCGGAACCCC AAAAAGCCGA 721 AACGGAAGCA GGATGAATAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 781 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 841 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACCAATCT AGTGATAACC 901 TAGCATGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt