Construct: ORF TRCN0000474503
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005973.1_s317c1
- Derived from:
- ccsbBroadEn_06071
- DNA Barcode:
- ATGAGCTTTTGGCAGTTCGACCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP2A6 (1548)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474503
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1548 | CYP2A6 | cytochrome P450 family 2 su... | NM_000762.6 | 99.7% | 100% | (many diffs) |
| 2 | human | 1549 | CYP2A7 | cytochrome P450 family 2 su... | NM_000764.2 | 96.4% | 93.7% | (many diffs) |
| 3 | human | 1553 | CYP2A13 | cytochrome P450 family 2 su... | NM_000766.5 | 95% | 93.5% | (many diffs) |
| 4 | human | 1549 | CYP2A7 | cytochrome P450 family 2 su... | NM_030589.2 | 86.1% | 83.4% | (many diffs) |
| 5 | human | 1549 | CYP2A7 | cytochrome P450 family 2 su... | XR_935754.3 | 24.8% | (many diffs) | |
| 6 | mouse | 13087 | Cyp2a5 | cytochrome P450, family 2, ... | NM_007812.4 | 84.5% | 85.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1551
- ORF length:
- 1482
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctggcctca gggatgcttc tggtggcctt gctggtctgc ctgactgtga 121 tggtcttgat gtctgtttgg cagcagagga agagcaaggg gaagctgcct ccgggaccca 181 ccccattgcc cttcattgga aactacctgc aactgaacac agagcagatg tacaactccc 241 tcatgaagat cagtgagcgc tatggccccg tgttcaccat tcacttgggg ccccggcggg 301 tcgtggtgct gtgtggacat gatgccgtca gggaggctct ggtggaccag gctgaggagt 361 tcagcgggcg aggcgagcaa gccaccttcg actgggtctt caaaggctat ggcgtggtat 421 tcagcaacgg ggagcgcgcc aagcagctcc ggcgcttctc catcgccacc ctgcgggact 481 tcggggtggg caagcgaggc atcgaggagc gcatccagga ggaggcgggc ttcctcatcg 541 acgccctccg gggcactggc ggcgccaata tcgatcccac cttcttcctg agccgcacag 601 tctccaatgt catcagctcc attgtctttg gggaccgctt tgactataag gacaaagagt 661 tcctgtcact gttgcgcatg atgctaggaa tcttccagtt cacgtcaacc tccacggggc 721 agctctatga gatgttctct tcggtgatga aacacctgcc aggaccacag caacaggcct 781 ttcagttgct gcaagggctg gaggacttca tagccaagaa ggtggagcac aaccagcgta 841 cgctggatcc caattcccca cgggacttca ttgactcctt tctcatccgc atgcaggagg 901 aggagaagaa ccccaacacg gagttctact tgaaaaacct ggtgatgacc acgttgaacc 961 tcttcattgg gggcaccgag accgtcagca ccaccctgcg ctatggcttc ttgctgctca 1021 tgaagcaccc agaggtggag gccaaggtcc atgaggagat tgacagagtg atcggcaaga 1081 accggcagcc caagtttgag gaccgggcca agatgcccta catggaggca gtgatccacg 1141 agatccaaag atttggagac gtgatcccca tgagtttggc ccgcagagtc aaaaaggaca 1201 ccaagtttcg ggatttcttc ctccctaagg gcaccgaagt gtaccctatg ctgggctctg 1261 tgctgagaga ccccagtttc ttctccaacc cccaggactt caatccccag catttcctga 1321 atgagaaggg gcagtttaag aagagtgatg cttttgtgcc cttttccatc ggaaagcgga 1381 actgtttcgg agaaggcctg gccagaatgg agctctttct cttcttcacc accgtcatgc 1441 agaacttccg cctcaagtcc tcccagtcac cTAAGGACAT TGACGTGTCC CCCAAACACG 1501 TGGGCTTTGC CACGATCCCA CGAAACTACA CCATGAGCTT CCTGCCCCGC TTGCCAACTT 1561 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1621 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1681 CTTGTGGAAA GGACGAATGA GCTTTTGGCA GTTCGACCCC ACGCGTTAAG TCgacaatca 1741 acctctggat tacaaaattt gtgaaagatt