Construct: ORF TRCN0000474509
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010571.1_s317c1
- Derived from:
- ccsbBroadEn_11791
- DNA Barcode:
- TAGATCGTTACGGAGTCTTCTTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUDT13 (25961)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474509
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25961 | NUDT13 | nudix hydrolase 13 | NM_001283014.2 | 100% | 100% | |
| 2 | human | 25961 | NUDT13 | nudix hydrolase 13 | NM_001283015.2 | 81.6% | 75.1% | (many diffs) |
| 3 | human | 25961 | NUDT13 | nudix hydrolase 13 | XM_017016033.2 | 81.6% | 75.1% | (many diffs) |
| 4 | human | 25961 | NUDT13 | nudix hydrolase 13 | NM_015901.6 | 74.7% | 74.7% | 592_858del |
| 5 | human | 25961 | NUDT13 | nudix hydrolase 13 | NM_001283016.1 | 38.9% | 38.9% | 0_1ins378;214_480del |
| 6 | human | 25961 | NUDT13 | nudix hydrolase 13 | XM_024447926.1 | 38.9% | 38.9% | 0_1ins378;214_480del |
| 7 | human | 25961 | NUDT13 | nudix hydrolase 13 | XR_001747079.2 | 38.8% | 1_121del;713_905del;1104_2030del | |
| 8 | human | 25961 | NUDT13 | nudix hydrolase 13 | NR_104264.2 | 36.8% | 1_122del;714_1018del;1217_2139del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 855
- ORF length:
- 789
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cctgtattgt ggaatagctt gcaggagaaa atttttttgg tgctataggc 121 tgctgtcaac ctatgttact aagacacggt atttatttga actgaaggaa gatgatgatg 181 catgtaaaaa agcccagcaa acaggagcgt tttacctctt tcatagtctg gctcctctgc 241 ttcagacttc agcacatcaa tacctggccc cccggcacag cctgttagag ttggaaaggc 301 tcctGGGTAA ATTTGGACAG GATGCACAAA GAATAGAAGA TTCTGTGCTG ATTGGATGCT 361 CTGAGCAGCA GGAAGCATGG TTTGCTCTGG ATCTAGGTCT GGATAGCTCC TTTTCCATAA 421 GTGCCTCCTT ACACAAACCT GAAATGGAGA CAGAGCTCAA GGGGTCTTTC ATTGAGCTGA 481 GAAAGGCACT CTTTCAACTC AATGCAAGGG ATGCCTCCTT GCTGTCCACG GCTCAAGCTC 541 TTCTCCGCTG GCATGATGCT CATCAGTTCT GCAGCAGAAG TGGGCAGCCC ACCAAGAAGA 601 ACGTGGCTGG CAGCAAGCGT GTGTGCCCTT CCAATAATAT AATCTATTAT CCACAGATCC 661 AGGTGAACTT GAGAGAATTA GAGACAGCTG CCTGGTTCAG TCATGATGAG GTAGCCACAG 721 CCCTGAAGAG AAAGGGCCCC TATACTCAGC AACAGAATGG GACTTTCCCA TTCTGGCTGC 781 CCCCTAAGTT AGCCATCTCC CACCAACTGA TTAAGGAGTG GGTGGAAAAA CAGACCTGTT 841 CTTCCCTGCC TGCTTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 901 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGCCCGTAA CTTGAAAGTA 961 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TAGATCGTTA CGGAGTCTTC 1021 TTCAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt