Construct: ORF TRCN0000474513
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016239.1_s317c1
- Derived from:
- ccsbBroadEn_04329
- DNA Barcode:
- TAATCCGACGAAGATGCCAAACAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARL6 (84100)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474513
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84100 | ARL6 | ADP ribosylation factor lik... | NM_001278293.3 | 100% | 100% | |
| 2 | human | 84100 | ARL6 | ADP ribosylation factor lik... | NM_032146.5 | 100% | 100% | |
| 3 | human | 84100 | ARL6 | ADP ribosylation factor lik... | NM_177976.3 | 100% | 100% | |
| 4 | human | 84100 | ARL6 | ADP ribosylation factor lik... | XM_017007311.2 | 100% | 100% | |
| 5 | human | 84100 | ARL6 | ADP ribosylation factor lik... | NM_001323513.2 | 96.3% | 91.7% | 536_548del;572_579delTGAAAAGA |
| 6 | human | 84100 | ARL6 | ADP ribosylation factor lik... | NM_001323514.2 | 85.4% | 85.4% | 477_478ins81 |
| 7 | human | 84100 | ARL6 | ADP ribosylation factor lik... | XM_017007312.2 | 85.4% | 85.4% | 477_478ins81 |
| 8 | human | 84100 | ARL6 | ADP ribosylation factor lik... | NR_136601.2 | 37.4% | 1_247del;806_1489del | |
| 9 | human | 84100 | ARL6 | ADP ribosylation factor lik... | NR_136600.2 | 37.1% | 1_247del;783_795del;819_1502del | |
| 10 | human | 84100 | ARL6 | ADP ribosylation factor lik... | NR_103511.2 | 35.1% | 1_346del;905_1588del | |
| 11 | human | 84100 | ARL6 | ADP ribosylation factor lik... | NR_136602.2 | 33.7% | 1_247del;725_726ins56;750_1433del | |
| 12 | human | 84100 | ARL6 | ADP ribosylation factor lik... | XR_001740321.2 | 16% | 1_2424del;2983_3487del | |
| 13 | human | 84100 | ARL6 | ADP ribosylation factor lik... | XR_001740319.2 | 15.8% | (many diffs) | |
| 14 | human | 84100 | ARL6 | ADP ribosylation factor lik... | XR_924187.3 | 14.9% | (many diffs) | |
| 15 | human | 84100 | ARL6 | ADP ribosylation factor lik... | XR_924189.3 | 14.6% | (many diffs) | |
| 16 | human | 84100 | ARL6 | ADP ribosylation factor lik... | NR_136598.2 | 13.9% | 1_247del;499_502delTAAG;810_3992del | |
| 17 | human | 84100 | ARL6 | ADP ribosylation factor lik... | NR_136597.2 | 12.2% | 1_247del;726_1282del;1363_4545del | |
| 18 | human | 84100 | ARL6 | ADP ribosylation factor lik... | NR_136595.2 | 11.9% | (many diffs) | |
| 19 | human | 84100 | ARL6 | ADP ribosylation factor lik... | XR_924184.3 | 8.8% | (many diffs) | |
| 20 | human | 84100 | ARL6 | ADP ribosylation factor lik... | XR_924188.3 | 8.7% | (many diffs) | |
| 21 | human | 84100 | ARL6 | ADP ribosylation factor lik... | XR_924185.3 | 8.6% | (many diffs) | |
| 22 | human | 84100 | ARL6 | ADP ribosylation factor lik... | XR_924186.3 | 8.5% | (many diffs) | |
| 23 | human | 84100 | ARL6 | ADP ribosylation factor lik... | XR_002959599.1 | 8.3% | 1_2483del;2962_3518del;3599_6665del | |
| 24 | mouse | 56297 | Arl6 | ADP-ribosylation factor-like 6 | NM_019665.3 | 89.4% | 95.6% | (many diffs) |
| 25 | mouse | 56297 | Arl6 | ADP-ribosylation factor-like 6 | NM_001347244.1 | 86.1% | 88.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 624
- ORF length:
- 558
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg attgctagac agactttcag tcttgcttgg cctGAAGAAG AAGGAGGTTC 121 ATGTTTTGTG CCTTGGGCTA GATAATAGTG GCAAAACGAC GATCATTAAC AAACTTAAAC 181 CTTCAAATGC TCAATCTCAA AATATCCTTC CAACAATAGG ATTCAGCATA GAGAAATTCA 241 AATCATCCAG TTTGTCATTT ACAGTGTTTG ACATGTCAGG TCAAGGAAGA TACAGAAATC 301 TCTGGGAACA CTATTATAAA GAAGGCCAAG CTATTATTTT TGTCATTGAT AGTAGTGATA 361 GATTAAGAAT GGTTGTGGCC AAAGAAGAAC TCGATACTCT TCTGAATCAT CCAGATATTA 421 AACACCGTCG AATTCCAATC TTATTCTTTG CAAATAAAAT GGATCTTAGA GATGCAGTGA 481 CATCTGTAAA AGTGTCTCAG TTGCTGTGTT TAGAGAACAT CAAAGATAAA CCCTGGCATA 541 TTTGTGCTAG TGATGCCATA AAAGGAGAAG GCTTGCAAGA AGGTGTAGAC TGGCTTCAAG 601 ATCAGATCCA GACTGTGAAG ACATGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 661 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 721 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT AATCCGACGA 781 AGATGCCAAA CACACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 841 att