Construct: ORF TRCN0000474514
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000969.1_s317c1
- Derived from:
- ccsbBroadEn_08133
- DNA Barcode:
- GTCATTAATATCCTCTCTTTTACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM216A (29902)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474514
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29902 | FAM216A | family with sequence simila... | NM_013300.3 | 99.8% | 100% | 762A>G |
| 2 | human | 29902 | FAM216A | family with sequence simila... | XM_011538246.3 | 83.3% | 83.1% | (many diffs) |
| 3 | human | 29902 | FAM216A | family with sequence simila... | XM_005253875.5 | 65.4% | 65.5% | 0_1ins282;480A>G |
| 4 | human | 29902 | FAM216A | family with sequence simila... | XM_024448959.1 | 65.4% | 65.5% | 0_1ins282;480A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 885
- ORF length:
- 819
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gggacagctg ctcccgcaca cggctcgcgg tctcggcgcc gcggagatgc 121 ccggccaggg tccggggtcc gactggacgg agcgtagctc ttctgcagag ccgcccgctg 181 tggccgggac cgagggtggc ggcggcggat cagctggata ctcttgttac cagaattcca 241 aaggttctga tagaatcaaa gatggataca aagtgaactc acacatagct aagctgcaag 301 agttatggaa aactccccaa aatcaaacaa tccacctctc taaatcaatg atggaggcgt 361 cctttttcaa gcatccagac ctcaccacag gccagaagcg ttacctgtgc agcattgcta 421 aaatctataa tgcaaactat ctgaagatgt taatgaagag gcagtacatg cacgtacttc 481 agcacagctc acaaaagcca ggtgTCCTCA CTCATCACAG AAGCCGCCTT AGCTCCCGTT 541 ACTCACAGAA ACAGCATTAC CCTTGCACTA CATGGCGACA TCAACTGGAG AGAGAGGACT 601 CGGGGTCTTC TGATATCGCA GCTGCATCTG CACCTGAAAT GCTCATACAG CATTCCCTTT 661 GGCGGCCAGT GAGAAACAAA GAAGGGATAA AAACTGGATA TGCATCTAAA ACAAGATGTA 721 AGTCACTGAA GATTTTTAGA AGACCAAGGA AACTGTTCAT GCAAACAGTT TCTTCAGATG 781 ATTCTGAATC ACACATGAGT GAAGAAAAAA AGGAAGAAGA TTTACTGAAT AATTTTATGC 841 AATCAATGTC AATTGAAGAA CAGGGAGAAC ATCTGATGTT AACTTGCCCA ACTTTCTTGT 901 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 961 AGTAATGAAC TAGCCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1021 GAAAGGACGA GTCATTAATA TCCTCTCTTT TACCACGCGT TAAGTCgaca atcaacctct 1081 ggattacaaa atttgtgaaa gatt