Construct: ORF TRCN0000474515
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005052.1_s317c1
- Derived from:
- ccsbBroadEn_12891
- DNA Barcode:
- TATCTTCCGATGTCTTGCTAACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRPF38A (84950)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474515
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84950 | PRPF38A | pre-mRNA processing factor 38A | NM_032864.4 | 40% | 40% | 1_561del |
| 2 | human | 84950 | PRPF38A | pre-mRNA processing factor 38A | XM_011542315.3 | 39.1% | 39.1% | 1_561del;748_768del |
| 3 | mouse | 230596 | Prpf38a | PRP38 pre-mRNA processing f... | NM_172697.3 | 33.8% | 39.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 441
- ORF length:
- 375
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGA TGATGTGGAG TCCAGTGAAG AGGAAGAAGA GGAGGATGAG AAGTTGGAAA 121 GAGTGCCATC ACCTGATCAC CGCCGGAGAA GCTACCGAGA CTTGGACAAG CCCCGTCGCT 181 CTCCCACACT GCGCTACAGG AGGAGTAGGA GCCGGTCTCC CAGAAGGCGG AGTCGATCTC 241 CCAAAAGGAG AAGCCCCTCC CCTCGCCGAG AAAGGCATCG GAGCAAGAGT CCAAGACGTC 301 ACCGCAGCAG GTCCCGAGAT CGGCGGCACA GATCCCGTTC CAAGTCCCCA GGTCATCACC 361 GTAGTCACAG ACACAGGAGC CACTCAAAGT CTCCCGAAAG GTCTAAGAAG AGCCACAAGA 421 AGAGCCGGAG AGGGAATGAG TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 481 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 541 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATATC TTCCGATGTC 601 TTGCTAACTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt