Construct: ORF TRCN0000474515

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005052.1_s317c1
Derived from:
ccsbBroadEn_12891
DNA Barcode:
TATCTTCCGATGTCTTGCTAACTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRPF38A (84950)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474515
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84950 PRPF38A pre-mRNA processing factor 38A NM_032864.4 40% 40% 1_561del
2 human 84950 PRPF38A pre-mRNA processing factor 38A XM_011542315.3 39.1% 39.1% 1_561del;748_768del
3 mouse 230596 Prpf38a PRP38 pre-mRNA processing f... NM_172697.3 33.8% 39.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
441
ORF length:
375
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattGATC AACAAGTTTG TACAAAAAAG
61TTGGCATGGA TGATGTGGAG TCCAGTGAAG AGGAAGAAGA GGAGGATGAG AAGTTGGAAA
121GAGTGCCATC ACCTGATCAC CGCCGGAGAA GCTACCGAGA CTTGGACAAG CCCCGTCGCT
181CTCCCACACT GCGCTACAGG AGGAGTAGGA GCCGGTCTCC CAGAAGGCGG AGTCGATCTC
241CCAAAAGGAG AAGCCCCTCC CCTCGCCGAG AAAGGCATCG GAGCAAGAGT CCAAGACGTC
301ACCGCAGCAG GTCCCGAGAT CGGCGGCACA GATCCCGTTC CAAGTCCCCA GGTCATCACC
361GTAGTCACAG ACACAGGAGC CACTCAAAGT CTCCCGAAAG GTCTAAGAAG AGCCACAAGA
421AGAGCCGGAG AGGGAATGAG TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
481CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
541AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATATC TTCCGATGTC
601TTGCTAACTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)