Construct: ORF TRCN0000474527
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003680.1_s317c1
- Derived from:
- ccsbBroadEn_06305
- DNA Barcode:
- TCCGAAAATTGTGCCTTAACGAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPLD1 (2822)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474527
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2822 | GPLD1 | glycosylphosphatidylinosito... | NM_001503.4 | 20.5% | 19.6% | (many diffs) |
| 2 | human | 2822 | GPLD1 | glycosylphosphatidylinosito... | XM_017010753.2 | 19.8% | 18.9% | (many diffs) |
| 3 | human | 2822 | GPLD1 | glycosylphosphatidylinosito... | XR_002956277.1 | 14% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 594
- ORF length:
- 528
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgctttcagg ttgtggcctg gcctgctgat catgttgggt tctctctgcc 121 atagaggttc accgtgtggc ctttcaacac acatagaaat aggacacaga gctctggagt 181 ttcttcagct tcacaatggg cgtgttaact acagagagct gttactagaa caccaggatg 241 cgtatcaggc tggaatcgtg tttcctgatt gtttttaccc TAGCATCTGC AAAGGAGGAA 301 AATTCCATGA TGTGTCTGAG AGCACTCACT GGACTCCGTT TCTTAATGCA AGCGTTCATT 361 ATATCCGAGA GAACTATCCC CTTCCCTGGG AGAAGGACAC AGAGAAACTG GTAGCTTTCT 421 TGTTTGGAAT TACTTCTCAC ATGGCGGCAG ATGTCAGCTG GCATAGTCTG GGCCTTGAAC 481 AAGGATTCCT TAGGACCATG GGAGCTATTG ATTTTCACGG CTCCTATTCA GAGGCTCATT 541 CGGCTGGTGA TTTTGGTACT GTTTATTTAC ATTTGCTAAA TTTTCTCGTG GTATACCCAA 601 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 661 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 721 TATCTTGTGG AAAGGACGAT CCGAAAATTG TGCCTTAACG AGCACGCGTT AAGTCgacaa 781 tcaacctctg gattacaaaa tttgtgaaag att