Construct: ORF TRCN0000474535
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018039.1_s317c1
- Derived from:
- ccsbBroadEn_13584
- DNA Barcode:
- GGGGACCAAAAATATGAGCCTGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- METTL2A (339175)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474535
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 339175 | METTL2A | methyltransferase like 2A | NM_181725.3 | 63.8% | 63.7% | 1_408del;435A>G;464T>C |
| 2 | human | 55798 | METTL2B | methyltransferase like 2B | NM_018396.2 | 63.3% | 63.4% | (many diffs) |
| 3 | human | 339175 | METTL2A | methyltransferase like 2A | XM_017024572.2 | 38.1% | 35.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 792
- ORF length:
- 726
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agaacagcac aagtgttctt cgaagagcct tgaacataaa acacagacac 121 ctcctgtgga ggagaatgta actcagaaaa ttagtgacct ggaaatttgt gctgatgagt 181 ttcctggatc ctcagccacc taccgaatac tggaggTTGG CTGTGGTGTG GGAAACACAG 241 TCTTTCCAAT TTTACAAACG AACAATGACC CAGGACTCTT TGTTTATTGC TGTGATTTTT 301 CTTCCACAGC TATAGAACTG GTCCAGACAA ATTCAGAATA TGATCCTTCT CGGTGTTTTG 361 CCTTTGTTCA CGACCTGTGT GATGAAGAGA AGAGTTACCC AGTGCCCAAG GGCAGTCTTG 421 ATATTATCAT TCTCATATTT GTTCTTTCAG CAATTGTTCC AGACAAGATG CAGAAGGCTA 481 TCAACAGGCT GAGCAGGCTT CTGAAACCTG GCGGGATGAT GCTTCTGCGA GATTACGGCC 541 GCTATGACAT GGCTCAGCTT CGGTTTAAAA AAGGTCAGTG TCTATCTGGA AATTTCTACG 601 TGAGAGGTGA TGGAACCAGA GTTTACTTCT TCACACAAGA GGAACTGGAC ACGCTTTTCA 661 CCACTGCTGG ACTGGAAAAA GTTCAGAACC TGGTGGATCG CCGACTGCAG GTGAACCGAG 721 GAAAGCAACT GACAATGTAC CGGGTTTGGA TTCAGTGCAA ATACTGCAAG CCCCTTCTGT 781 CCAGCACCAG CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 841 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 901 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGGG GACCAAAAAT ATGAGCCTGA 961 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t