Construct: ORF TRCN0000474536
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003886.1_s317c1
- Derived from:
- ccsbBroadEn_00688
- DNA Barcode:
- ACTTCTCGATTAAGGTGTTACTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRB2 (2885)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474536
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2885 | GRB2 | growth factor receptor boun... | NM_002086.5 | 100% | 100% | |
| 2 | human | 2885 | GRB2 | growth factor receptor boun... | NM_203506.2 | 81.1% | 81.1% | 176_177ins123 |
| 3 | mouse | 14784 | Grb2 | growth factor receptor boun... | NM_001313936.1 | 92.1% | 99.5% | (many diffs) |
| 4 | mouse | 14784 | Grb2 | growth factor receptor boun... | NM_001313937.1 | 92.1% | 99.5% | (many diffs) |
| 5 | mouse | 14784 | Grb2 | growth factor receptor boun... | NM_008163.4 | 92.1% | 99.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 717
- ORF length:
- 651
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agccatcgcc aaatatgact tcaaagctac tgcagacgac gagctgagct 121 tcaaaagggg ggacatcctc aaggttttGA ACGAAGAATG TGATCAGAAC TGGTACAAGG 181 CAGAGCTTAA TGGAAAAGAC GGCTTCATTC CCAAGAACTA CATAGAAATG AAACCACATC 241 CGTGGTTTTT TGGCAAAATC CCCAGAGCCA AGGCAGAAGA AATGCTTAGC AAACAGCGGC 301 ACGATGGGGC CTTTCTTATC CGAGAGAGTG AGAGCGCTCC TGGGGACTTC TCCCTCTCTG 361 TCAAGTTTGG AAACGATGTG CAGCACTTCA AGGTGCTCCG AGATGGAGCC GGGAAGTACT 421 TCCTCTGGGT GGTGAAGTTC AATTCTTTGA ATGAGCTGGT GGATTATCAC AGATCTACAT 481 CTGTCTCCAG AAACCAGCAG ATATTCCTGC GGGACATAGA ACAGGTGCCA CAGCAGCCGA 541 CATACGTCCA GGCCCTCTTT GACTTTGATC CCCAGGAGGA TGGAGAGCTG GGCTTCCGCC 601 GGGGAGATTT TATCCATGTC ATGGATAACT CAGACCCCAA CTGGTGGAAA GGAGCTTGCC 661 ACGGGCAGAC CGGCATGTTT CCCCGCAATT ATGTCACCCC CGTGAACCGG AACGTCTACC 721 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 781 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 841 ATATATCTTG TGGAAAGGAC GAACTTCTCG ATTAAGGTGT TACTTAACGC GTTAAGTCga 901 caatcaacct ctggattaca aaatttgtga aagatt