Construct: ORF TRCN0000474573
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015768.1_s317c1
- Derived from:
- ccsbBroadEn_13015
- DNA Barcode:
- GAACGCGATGCCACTCCGAGACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTFR2 (113115)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474573
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 113115 | MTFR2 | mitochondrial fission regul... | NM_001318738.2 | 65.4% | 65.4% | 1_354del |
| 2 | human | 113115 | MTFR2 | mitochondrial fission regul... | XM_011535410.2 | 65.4% | 65.4% | 1_354del |
| 3 | human | 113115 | MTFR2 | mitochondrial fission regul... | XM_011535412.2 | 65.4% | 65.4% | 1_354del |
| 4 | human | 113115 | MTFR2 | mitochondrial fission regul... | XM_011535413.2 | 65.4% | 65.4% | 1_354del |
| 5 | human | 113115 | MTFR2 | mitochondrial fission regul... | NM_001099286.3 | 58.1% | 58.1% | 1_483del |
| 6 | human | 113115 | MTFR2 | mitochondrial fission regul... | NM_138419.5 | 58.1% | 58.1% | 1_483del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 738
- ORF length:
- 672
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ggaactgaaa aatagtacaa attctagttc ctttggcttg agtgacgagc 121 gcattagttt gggtcagctg tcatcatcgc gggctgccca tctgagtgtg gacccagatc 181 agcttccagg ttcagtgctt tctcctcctc ctcctccacc acttcctcct cagttttcat 241 ctctccagcc accgtgtttt ccTCCCGTAC AACCAGGATC TAATAATATT TGTGACTCAG 301 ATAATCCAGC AACTGAAATG AGCAAACAGA ACCCGGCTGC TAATAAGACC AATTATAGTC 361 ATCATTCAAA AAGCCAGAGA AATAAAGATA TTCCAAACAT GTTGGACGTT CTAAAGGATA 421 TGAATAAGGT TAAGCTTCGT GCAATTGAGC GGTCACCTGG CGGTAGACCC ATTCATAAGA 481 GGAAAAGACA GAATTCACAT TGGGATCCAG TTTCTTTAAT ATCTCATGCA CTTAAACAGA 541 AATTTGCATT TCAAGAAGAT GATTCTTTTG AGAAAGAGAA TAGATCTTGG GAATCTTCCC 601 CATTTTCTAG TCCAGAAACT TCAAGGTTTG GACATCACAT TTCACAGTCA GAAGGACAGC 661 GAACTAAAGA AGAAATGGTC AACACAAAAG CTGTTGACCA AGGTATCAGC AACACAAGCC 721 TTCTAAACTC AAGGATTTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 781 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 841 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGAACGCG ATGCCACTCC 901 GAGACTCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt