Construct: ORF TRCN0000474574
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010534.1_s317c1
- Derived from:
- ccsbBroadEn_01744
- DNA Barcode:
- AAACTAGGACTTTAAATCCGTCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UCHL1 (7345)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474574
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7345 | UCHL1 | ubiquitin C-terminal hydrol... | NM_004181.5 | 100% | 100% | |
| 2 | mouse | 22223 | Uchl1 | ubiquitin carboxy-terminal ... | NM_011670.2 | 88% | 95.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 735
- ORF length:
- 669
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gctcaagccg atggagatca accccgagat gctgaacaaa gtgctgtccc 121 ggctgggggt cgccggccag tggcgcttcg tggacgtgct ggggctggaa gaggagtctc 181 tgggctcggt gccagcgcct gcctgcgcgc tgctgctgct gtttcccctc acggcccagc 241 atgagaactt caggaaaaag cagattgaag agctgaaggg acaagaagtt agtccTAAAG 301 TGTACTTCAT GAAGCAGACC ATTGGGAATT CCTGTGGCAC AATCGGACTT ATTCACGCAG 361 TGGCCAATAA TCAAGACAAA CTGGGATTTG AGGATGGATC AGTTCTGAAA CAGTTTCTTT 421 CTGAAACAGA GAAAATGTCC CCTGAAGACA GAGCAAAATG CTTTGAAAAG AATGAGGCCA 481 TACAGGCAGC CCATGATGCC GTGGCACAGG AAGGCCAATG TCGGGTAGAT GACAAGGTGA 541 ATTTCCATTT TATTCTGTTT AACAACGTGG ATGGCCACCT CTATGAACTT GATGGACGAA 601 TGCCTTTTCC GGTGAACCAT GGCGCCAGTT CAGAGGACAC CCTGCTGAAG GACGCTGCCA 661 AGGTCTGCAG AGAATTCACC GAGCGTGAGC AAGGAGAAGT CCGCTTCTCT GCCGTGGCTC 721 TCTGCAAGGC AGCCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 781 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 841 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AAACTAGGAC TTTAAATCCG 901 TCAGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt