Transcript: Human NM_004181.5

Homo sapiens ubiquitin C-terminal hydrolase L1 (UCHL1), mRNA.

Source:
NCBI, updated 2019-09-22
Taxon:
Homo sapiens (human)
Gene:
UCHL1 (7345)
Length:
1103
CDS:
50..721

Additional Resources:

NCBI RefSeq record:
NM_004181.5
NBCI Gene record:
UCHL1 (7345)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_004181.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000007275 CTGTGGCACAATCGGACTTAT pLKO.1 316 CDS 100% 13.200 18.480 N UCHL1 n/a
2 TRCN0000011079 CAGTTCTGAAACAGTTTCTTT pLKO.1 384 CDS 100% 5.625 3.938 N UCHL1 n/a
3 TRCN0000318429 CAGTTCTGAAACAGTTTCTTT pLKO_005 384 CDS 100% 5.625 3.938 N UCHL1 n/a
4 TRCN0000007274 CGGGTAGATGACAAGGTGAAT pLKO.1 506 CDS 100% 4.950 3.465 N UCHL1 n/a
5 TRCN0000349547 CGGGTAGATGACAAGGTGAAT pLKO_005 506 CDS 100% 4.950 3.465 N UCHL1 n/a
6 TRCN0000007273 GTGTGAGCTTCAGATGGTGAA pLKO.1 917 3UTR 100% 4.050 2.835 N UCHL1 n/a
7 TRCN0000349548 GTGTGAGCTTCAGATGGTGAA pLKO_005 917 3UTR 100% 4.050 2.835 N UCHL1 n/a
8 TRCN0000007276 CCAGCATGAGAACTTCAGGAA pLKO.1 220 CDS 100% 2.640 1.848 N UCHL1 n/a
9 TRCN0000318428 CCAGCATGAGAACTTCAGGAA pLKO_005 220 CDS 100% 2.640 1.848 N UCHL1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_004181.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01744 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_01744 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000474574 AAACTAGGACTTTAAATCCGTCAG pLX_317 65.3% 100% 100% V5 n/a
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