Construct: ORF TRCN0000474589
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015953.1_s317c1
- Derived from:
- ccsbBroadEn_09606
- DNA Barcode:
- ATCCACAATATTTTAACATGATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIWIL4 (143689)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474589
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 143689 | PIWIL4 | piwi like RNA-mediated gene... | NM_152431.3 | 99.9% | 100% | 1920C>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2622
- ORF length:
- 2556
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tggaagagcc cgagtgaagg ccagaggcat cgcccgcagc cccagtgcca 121 cagaagtggg gcgcatccaa gcctcgccat tgcctagatc tgttgatctt agtaacaatg 181 aagcatcctc tagcaatggc ttcttgggaa caagcaggat ctcaaccaac gataaatatg 241 ggatatcttc tggtgatgct ggaagtacct tcatggaaag aggtgtgaaa aacaaacagg 301 actttatgga tttgagtatc tgtaccagag aaaaattggc acatgtgaga aattgtaaaa 361 caggttccag tggaatacct gtgaaactgg ttacaaacct ctttaactta gattttcccc 421 aagactggca gctataccag taccatgtga catatattcc agatttagca tctagaaggc 481 tgagaattgc tttactttat agtcatagtg aactttccaa caaagcaaaa gcattcgacg 541 gtgccatcct ttttctgtca caaaagctag aagaaaaggt cacagagttg tcaagtgaaa 601 ctcaaagagg tgagactata aagatgacta tcaccctgaa gagggagctg ccatcaagtt 661 ctcccgtgtg catccaggtc ttcaatatca tcttcagaaa gatcctcaaa aagttgtcca 721 tgtaccaaat tggacggaac ttctataatc cttcagagcc aatggaaatt ccccagcaca 781 aattatccct ttggcctggg tttgccattt ctgtgtcata ttttgaaagg aagctcctgt 841 ttagtgctga tgtgagttac aaagtcctcc ggaatgagac ggttctggaa ttcatgactg 901 ctctctgtca aagaactggc ttgtcctgtt tcacccagac gtgtgagaag cagctaatag 961 ggctcattgt ccttacaaga tacaataaca gaacctactc cattgatgac attgactggt 1021 cagtgaagcc cacacacacc tttcagaagc gggatggcac cgagatcacc tatgtggatt 1081 actacaagca gcagtatgat attactgtat cggacctgaa tcagcccatg cttgttagtc 1141 tgttaaagaa gaagagaaat gacaacagtg aggctcagct cgcccacctg atacctgagc 1201 tctgctttct aacagggctg actgaccagg caacatctga tttccagctg atgaaggctg 1261 tggctgaaaa gacacgtctc agtccttcag gccggcagca gcgcctggcc aggcttgtgg 1321 acaacatcca gaggaatacc aatgctcgct ttgaactaga gacctgggga ctgcattttg 1381 gaagccagat atctctgact ggccggattg tgccttcaga aaaaatatta atgcaagacc 1441 acatatgtca acctgtgtct gctgctgact ggtccaagga tattcgaact tgcaagattt 1501 taaatgcaca gtctttgaat acctggttga ttttatgtag cgacagaact gaatatgttg 1561 ccgagagctt tctgaactgc ttgagaagag ttgcaggttc catgggattt aatgtggact 1621 accccaaaat cataaaagta caagaaaatc cagctgcatt tgttagagct atacagcaat 1681 atgttgatcc tgatgttcag ctggtaatgt gcattctgcc ttctaatcag aagacctatt 1741 atgattccat taaaaaatat ttgagctcag actgcccagt cccaagccaa tgtgtgcttg 1801 ctcggacctt gaataaacag ggcatgatga tgagtatcgc caccaagatc gctatgcaga 1861 tgacttgcaa gctcggaggc gagctgtggg ctgtggaaat acctttaaag tccctgatgg 1921 tggtcggtat tgatgtctgt aaagatgcac tcagcaagga cgtgatggtt gttggatgcg 1981 tggcaagtgt taaccccaga atcaccaggt ggttttcccg ctgtatcctt cagagaacaa 2041 tgactgatgt tgcagattgc ttgaaagttt tcatgactgg agcactcaac aaatggtaca 2101 agtacaaTCA TGATTTGCCA GCACGGATAA TTGTGTACCG TGCTGGTGTA GGGGATGGTC 2161 AGCTGAAAAC ACTTATTGAA TATGAAGTCC CACAGCTGCT GAGCAGTGTG GCAGAATCCA 2221 GCTCAAATAC CAGCTCAAGA CTGTCGGTGA TTGTGGTCAG GAAGAAGTGC ATGCCACGAT 2281 TCTTTACCGA AATGAACCGC ACTGTACAGA ACCCCCCACT TGGCACTGTT GTGGATTCAG 2341 AAGCAACACG TAACGAATGG TATGACTTTT ATCTGATCAG CCAGGTGGCC TGCCGGGGAA 2401 CTGTTAGTCC TACCTACTAT AATGTCATCT ATGATGACAA CGGCTTGAAG CCCGACCATA 2461 TGCAGAGACT TACATTCAAA TTGTGCCACC TGTACTACAA CTGGCCGGGC ATAGTCAGTG 2521 TCCCAGCACC ATGTCAGTAT GCTCACAAGC TGACCTTTCT GGTGGCACAA AGCATTCATA 2581 AAGAACCCAG TCTGGAATTA GCCAACCATC TCTTCTACCT GTGCCCAACT TTCTTGTACA 2641 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2701 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2761 AGGACGAATC CACAATATTT TAACATGATT CACGCGTTAA GTCgacaatc aacctctgga 2821 ttacaaaatt tgtgaaagat t