Construct: ORF TRCN0000474592
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005913.1_s317c1
- Derived from:
- ccsbBroadEn_03654
- DNA Barcode:
- GTGCGCGTACCGCTTAACCACACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SAYSD1 (55776)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474592
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55776 | SAYSD1 | SAYSVFN motif domain contai... | NM_018322.3 | 100% | 100% | |
| 2 | human | 55776 | SAYSD1 | SAYSVFN motif domain contai... | NM_001304793.2 | 62.8% | 62.2% | 1_1delAins202;3G>T;5T>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 615
- ORF length:
- 549
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttG TACAAAAAAG 61 TTGGCATGGA ACAGCGGTTA GCTGAGTTTC GGGCGGCGCG GAAACGGGCG GGTCTGGCGG 121 CCCAACCCCC TGCTGCCAGT CAGGGCGCAC AAACCCCAGG AGAGAAGGCG GAAGCAGCAG 181 CGACTCTAAA GGCAGCCCCA GGCTGGCTAA AGCGGTTCCT GGTATGGAAA CCTAGGCCCG 241 CGAGTGCCCG GGCCCAGCCC GGCCTAGTTC AGGAAGCGGC TCAGCCCCAG GGCAGCACAT 301 CAGAGACACC ATGGAACACA GCCATTCCTC TGCCGTCGTG CTGGGACCAG TCTTTCCTGA 361 CCAATATCAC CTTCTTGAAG GTTCTTCTCT GGTTGGTCCT GCTGGGACTG TTTGTGGAAC 421 TGGAATTTGG CCTGGCATAT TTTGTCCTGT CCTTGTTCTA TTGGATGTAC GTCGGGACAC 481 GAGGCCCTGA AGAGAAGAAA GAGGGAGAGA AGAGCGCCTA CTCTGTGTTC AATCCAGGCT 541 GTGAAGCCAT CCAGGGCACC CTGACTGCAG AGCAGTTGGA GCGCGAGTTA CAGTTGAGAC 601 CCCTGGCAGG GAGATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 661 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 721 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GTGCGCGTAC CGCTTAACCA 781 CACTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt