Construct: ORF TRCN0000474597
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015453.1_s317c1
- Derived from:
- ccsbBroadEn_05910
- DNA Barcode:
- TATTGGAGCCTTCCCACGGCGGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SERPING1 (710)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474597
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 710 | SERPING1 | serpin family G member 1 | NM_000062.3 | 99.9% | 99.8% | 1438G>A |
| 2 | human | 710 | SERPING1 | serpin family G member 1 | NM_001032295.2 | 99.9% | 99.8% | 1438G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1566
- ORF length:
- 1500
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctccaggctg accctgctga ccctcctgct gctgctgctg gctggggata 121 gagcctcctc aaatccaaat gctaccagct ccagctccca ggatccagag agtttgcaag 181 acagaggcga agggaaggtc gcaacaacag ttatctccaa gatgctattc gttgaaccca 241 tcctggaggt ttccagcttg ccgacaacca actcaacaac caattcagcc accaaaataa 301 cagctaatac cactgatgaa cccaccacac aacccaccac agagcccacc acccaaccca 361 ccatccaacc cacccaacca actacccagc tcccaacaga ttctcctacc cagcccacta 421 ctgggtcctt ctgcccagga cctgttactc tctgctctga cttggagagt cattcaacag 481 aggccgtgtt gggggatgct ttggtagatt tctccctgaa gctctaccac gccttctcag 541 caatgaagaa ggtggagacc aacatggcct tttccccatt cagcatcgcc agcctcctta 601 cccaggtcct gctcggggct ggggagaaca ccaaaacaaa cctggagagc atcctctctt 661 accccaagga cttcacctgt gtccaccagg ccctgaaggg cttcacgacc aaaggtgtca 721 cctcagtctc tcagatcttc cacagcccag acctggccat aagggacacc tttgtgaatg 781 cctctcggac cctgtacagc agcagcccca gagtcctaag caacaacagt gacgccaact 841 tggagctcat caacacctgg gtggccaaga acaccaacaa caagatcagc cggctgctag 901 acagtctgcc ctccgatacc cgccttgtcc tcctcaatgc tatctacctg agtgccaagt 961 ggaagacaac atttgatccc aagaaaacca gaatggaacc ctttcacttc aaaaactcag 1021 ttataaaagt gcccatgatg aatagcaaga agtaccctgt ggcccatttc attgaccaaa 1081 ctttgaaagc caaggtgggg cagctgcagc tctcccacaa tctgagtttg gtgatcctgg 1141 taccccagaa cctgaaacat cgtcttGAAG ACATGGAACA GGCTCTCAGC CCTTCTGTTT 1201 TCAAGGCCAT CATGGAGAAA CTGGAGATGT CCAAGTTCCA GCCCACTCTC CTAACACTAC 1261 CCCGCATCAA AGTGACGACC AGCCAGGATA TGCTCTCAAT CATGGAGAAA TTGGAATTCT 1321 TCGATTTTTC TTATGACCTT AACCTGTGTG GGCTGACAGA GGACCCAGAT CTTCAGGTTT 1381 CTGCGATGCA GCACCAGACA GTGCTGGAAC TGACAGAGAC TGGGGTGGAG GCGGCTGCAG 1441 CCTCCGCCAT CTCTGTGGCC CGCACCCTGC TGGTCTTTGA AGTGCAGCAG CCCTTCCTCT 1501 TCATGCTCTG GGACCAGCAG CACAAGTTCC CTGTCTTCAT GGGGCGAGTA TATGACCCCA 1561 GGGCCTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1621 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1681 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATATTGGAGC CTTCCCACGG CGGACACGCG 1741 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt