Construct: ORF TRCN0000474616
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016194.1_s317c1
- Derived from:
- ccsbBroadEn_08322
- DNA Barcode:
- CTGAGTTCGTTCGTCTTATCAGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UFM1 (51569)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474616
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51569 | UFM1 | ubiquitin fold modifier 1 | NM_016617.4 | 99.6% | 100% | 240T>C |
| 2 | human | 51569 | UFM1 | ubiquitin fold modifier 1 | NM_001286703.2 | 97.2% | 97.6% | 59_60insACTCAG;234T>C |
| 3 | human | 51569 | UFM1 | ubiquitin fold modifier 1 | NM_001286705.2 | 78.9% | 77.6% | (many diffs) |
| 4 | human | 51569 | UFM1 | ubiquitin fold modifier 1 | NM_001286706.2 | 78.9% | 77.6% | (many diffs) |
| 5 | human | 51569 | UFM1 | ubiquitin fold modifier 1 | NM_001286704.2 | 71.9% | 65.4% | (many diffs) |
| 6 | human | 51569 | UFM1 | ubiquitin fold modifier 1 | NR_104584.2 | 7.8% | (many diffs) | |
| 7 | human | 51569 | UFM1 | ubiquitin fold modifier 1 | NR_104585.2 | 6.7% | (many diffs) | |
| 8 | mouse | 67890 | Ufm1 | ubiquitin-fold modifier 1 | NM_026435.5 | 90.1% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 92
- ORF end:
- 347
- ORF length:
- 255
- Sequence:
-
1 CCTCAGACAG TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG CTAGCATCGA 61 TTGATCAACA AGTTTGTACA AAAAAGTTGG CATGTCGAAG GTTTCCTTTA AGATCACGCT 121 GACGTCGGAC CCACGGCTGC CGTACAAAGT ACTCAGTGTT CCTGAAAGTA CACCTTTCAC 181 AGCAGTCTTA AAGTTTGCAG CAGAAGAATT TAAAGTTCCT GCTGCAACAA GTGCAATTAT 241 TACCAATGAT GGAATAGGAA TAAATCCTGC ACAGACTGCT GGAAATGTTT TTCTAAAACA 301 TGGTTCAGAA CTGCGGATTA TTCCTAGAGA CCGTGTTGGA AGTTGTTACC CAACTTTCTT 361 GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC 421 GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG 481 TGGAAAGGAC GACTGAGTTC GTTCGTCTTA TCAGGCACGC GTTAAGTCga caatcaacct 541 ctggattaca aaatttgtga aagatt