Construct: ORF TRCN0000474636
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018076.1_s317c1
- Derived from:
- ccsbBroadEn_02084
- DNA Barcode:
- ACGCTGGGGCCGTCCTATTACCTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SEC22C (9117)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474636
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | NM_032970.4 | 99.8% | 55.7% | 486_487insG |
| 2 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | XM_011534212.2 | 99.8% | 55.7% | 486_487insG |
| 3 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | XM_024453812.1 | 99.8% | 55.7% | 486_487insG |
| 4 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | XM_024453813.1 | 99.8% | 55.7% | 486_487insG |
| 5 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | NM_001201572.2 | 81.3% | 67.6% | (many diffs) |
| 6 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | NM_004206.4 | 81.3% | 67.6% | (many diffs) |
| 7 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | XM_024453814.1 | 81.3% | 67.6% | (many diffs) |
| 8 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | XM_024453815.1 | 81.3% | 67.6% | (many diffs) |
| 9 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | XM_005265533.1 | 76.8% | 32.6% | 0_1ins210;276_277insG |
| 10 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | XM_005265534.1 | 76.8% | 32.6% | 0_1ins210;276_277insG |
| 11 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | XM_005265535.1 | 76.8% | 32.6% | 0_1ins210;276_277insG |
| 12 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | XM_024453816.1 | 76.8% | 32.6% | 0_1ins210;276_277insG |
| 13 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | XM_024453817.1 | 76.8% | 32.6% | 0_1ins210;276_277insG |
| 14 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | NM_001201584.2 | 72.8% | 74.1% | (many diffs) |
| 15 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | XM_017007438.2 | 58.3% | 39.6% | (many diffs) |
| 16 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | XM_017007439.2 | 58.3% | 39.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 597
- ORF length:
- 531
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cgtgatcttt tttgcctgcg tggtacgggt aagggatgga ctgcccctct 121 cagcctctac tgatttttac cacacccaag attttttgga atggaggaga cggctcaaga 181 gtttagcctt gcgactggcc cagtatccag gtcgaggttc tgcagaaggt tgtgacttta 241 gtatacattt ttcttctttc ggggacgtgg cctgcatggc tatctgctcc tgccagtgtc 301 cagcagccat ggccttctgc ttcctggaga ccctgtggtg ggaattcaca gcttcctatg 361 acactacctg cattggccta gcctccaggc catacgcttt tcttgagttt gacagcatca 421 ttcagaaagt gaagtggcat tttaactatg taagttcctc TCAGATGGAG TGCAGCTTGG 481 AAAAAATTCA GGAGGAGCTC AAGTTGCAGC CTCCAGCGGT TCTCACTCTG GAGGACACAG 541 ATGTGGCAAA TGGGGGTGAT GAATGGTCAC ACACCGATGC ACTTGGAGCC TGCTCCTAAT 601 TTCCGAATGG AACCAGTGAC AGCCCTGGGT ATCCTCTCCC TCATTCTCAA CATCATGTGT 661 GCTGCCCTGA ATCTCATTCG AGGAGTTCAC CTTGCAGAAC ATTCTTTACA GGTTGCCCAT 721 GAGGAAATTG GAAACATTCT GGCTTTTCTT GTTCCTTTCG TAGCCTGCAT TTTCCAGTGT 781 TATTTGTACC TGTTCTACAG TCCAGCCAGG ACTATGAAGG TGGTGCTTAT GCTGCTCTTT 841 ATTTGCCTGG GCAACATGTA CCTGCACGGG CTGAGGAACC TCTGGCAAAT CCTTTTCCAC 901 ATAGGAGTGG CTTTTCTGTC TTCATATCAG ATACTAACAA GGCAGCTTCA GGAGAAGCAG 961 TCTGACTGTG GAGTATGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC 1021 CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT 1081 ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AACGCTGGGG CCGTCCTATT 1141 ACCTTACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt