Construct: ORF TRCN0000474640
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002491.1_s317c1
- Derived from:
- ccsbBroadEn_12293
- DNA Barcode:
- CCTACGAATGCTAGCCAAAACCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEPTIN3 (55964)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474640
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55964 | SEPTIN3 | septin 3 | NM_145733.3 | 96.3% | 96.3% | 1_39del |
| 2 | human | 55964 | SEPTIN3 | septin 3 | NM_019106.5 | 92% | 89.5% | (many diffs) |
| 3 | human | 55964 | SEPTIN3 | septin 3 | NM_001363845.2 | 40.3% | 40.3% | 1_1533del |
| 4 | mouse | 24050 | Sept3 | septin 3 | XM_017316607.1 | 90% | 96% | (many diffs) |
| 5 | mouse | 24050 | Sept3 | septin 3 | XM_017316608.1 | 89.7% | 95.8% | (many diffs) |
| 6 | mouse | 24050 | Sept3 | septin 3 | NM_011889.2 | 89% | 92.5% | (many diffs) |
| 7 | mouse | 24050 | Sept3 | septin 3 | XM_006520992.1 | 84.3% | 88.7% | (many diffs) |
| 8 | mouse | 24050 | Sept3 | septin 3 | XM_017316609.1 | 84.3% | 88.7% | (many diffs) |
| 9 | mouse | 24050 | Sept3 | septin 3 | XM_017316610.1 | 84% | 88.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1104
- ORF length:
- 1035
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcagagctg gtgcctgagc ccaggcctaa gccagcggtg cccatgaagc 121 ccatgagcat caactccaac ctgctgggct acatcggcat cgacaccatc atcgagcaga 181 tgcgcaagaa gaccatgaag accggtttcg acttcaacat catggtcgtt ggccagagtg 241 gactgggcaa atcaacgctg gtcaacacgc tcttcaaatc ccaagtgagc cgcaaggcct 301 ccagctggaa ccgggaggag aagatcccca agacagtgga gatcaaagct atcgggcatg 361 tgatagagga aggcggtgtc aaaatgaagc tgaccgtcat cgacacccca ggctttggag 421 accaaatcaa caatgaaaac tgctgggagc ccattgagaa gtacatcaat gagcagtacg 481 agaagttcct gaaggaggag gtcaacatcg ccaggaagaa acgcatccct gacactcgtg 541 tccactgctg cctttacttc atctctccca caggacactc cttgcgacct ctggatcttg 601 agttcatgaa acacctcagc aaggttgtga acatcatccc tgtcattgct aaggctgaca 661 ccatgaccct GGAGGAGAAG TCTGAATTCA AGCAAAGGGT TCGCAAGGAG CTTGAAGTAA 721 ATGGCATTGA ATTCTACCCC CAGAAGGAAT TTGATGAGGA TTTGGAGGAT AAGACGGAGA 781 ATGACAAAAT CAGGCAGGAG AGCATGCCTT TTGCTGTGGT GGGAAGTGAC AAGGAGTACC 841 AAGTGAATGG CAAGAGGGTC CTCGGCCGAA AAACTCCATG GGGGATCATC GAAGTGGAAA 901 ACCTCAACCA CTGTGAGTTT GCCCTGCTTC GAGACTTTGT CATCAGGACC CACCTCCAGG 961 ACCTCAAGGA AGTGACACAC AACATCCACT ATGAGACTTA CAGGGCCAAG CGGCTCAATG 1021 ACAATGGAGG CCTCCCTCCG GGAGAAGGCC TCCTGGGCAC TGTCCTTCCA CCTGTGCCAG 1081 CCACCCCCTG CCCCACTGCT GAATTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1141 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1201 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC CTACGAATGC 1261 TAGCCAAAAC CCCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1321 att