Construct: ORF TRCN0000474641
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000886.1_s317c1
- Derived from:
- ccsbBroadEn_08187
- DNA Barcode:
- CACTTAGAGAGCGAGCTATGGTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAS2R14 (50840)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474641
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50840 | TAS2R14 | taste 2 receptor member 14 | NM_023922.1 | 99.7% | 99.6% | 375G>A;929A>C |
| 2 | human | 106707243 | PRH1-TAS2R14 | PRH1-TAS2R14 readthrough | NM_001316893.2 | 45% | 38.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1020
- ORF length:
- 951
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggtggtgtc ataaagagca tatttacatt cgttttaatt gtggaattta 121 taattggaaa tttaggaaat agtttcatag cactggtgaa ctgtattgac tgggtcaagg 181 gaagaaagat ctcttcggtt gatcggatcc tcactgcttt ggcaatctct cgaattagcc 241 tggtttggtt aatattcgga agctggtgtg tgtctgtgtt tttcccagct ttatttgcca 301 ctgaaaaaat gttcagaatg cttactaata tctggacagt gatcaatcat tttagtgtct 361 ggttagctac aggcctcggt actttttatt ttctcaagat agccaatttt tctaactcta 421 tttttctcta cctaaagtgg agagttaaaa aggtggtttt ggtgctgctt cttgtgactt 481 cggtcttctt gtttttaaat attgcactga taaacatcca tataaatgcc agtatcaatg 541 gatacagaag aaaCAAGACT TGCAGTTCTG ATTCAAGTAA CTTTACACGA TTTTCCAGTC 601 TTATTGTATT AACCAGCACT GTGTTCATTT TCATACCCTT TACTTTGTCC CTGGCAATGT 661 TTCTTCTCCT CATCTTCTCC ATGTGGAAAC ATCGCAAGAA GATGCAGCAC ACTGTCAAAA 721 TATCCGGAGA CGCCAGCACC AAAGCCCACA GAGGAGTTAA AAGTGTGATC ACTTTCTTCC 781 TACTCTATGC CATTTTCTCT CTGTCTTTTT TCATATCAGT TTGGACCTCT GAAAGGTTGG 841 AGGAAAATCT AATTATTCTT TCCCAGGTGA TGGGAATGGC TTATCCTTCA TGTCACTCAT 901 GTGTTCTGAT TCTTGGAAAC AAGAAGCTGA GACAGGCCTC TCTGTCAGTG CTACTGTGGC 961 TGAGGTACAT GTTCAAAGAT GGGGAGCCCT CAGGTCCCAA AGAATTTAGA GAATCATCTT 1021 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1081 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1141 TTTATATATC TTGTGGAAAG GACGACACTT AGAGAGCGAG CTATGGTCCA CGCGTTAAGT 1201 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt