Construct: ORF TRCN0000474642
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011739.1_s317c1
- Derived from:
- ccsbBroadEn_04227
- DNA Barcode:
- TCTTCTGGATACGAGTGATTTGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SETD7 (80854)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474642
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80854 | SETD7 | SET domain containing 7, hi... | NM_030648.3 | 100% | 100% | |
| 2 | human | 80854 | SETD7 | SET domain containing 7, hi... | NM_001306199.1 | 88.1% | 84% | (many diffs) |
| 3 | human | 80854 | SETD7 | SET domain containing 7, hi... | XM_017008661.1 | 62.2% | 62.2% | 0_1ins414 |
| 4 | human | 80854 | SETD7 | SET domain containing 7, hi... | NM_001306200.1 | 36.1% | 34.1% | (many diffs) |
| 5 | mouse | 73251 | Setd7 | SET domain containing (lysi... | NM_080793.5 | 87.9% | 95.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1167
- ORF length:
- 1098
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatagcgac gacgagatgg tggaggaggc ggtggaaggg cacctggacg 121 atgacggatt accgcacggg ttctgcacag tcacctactc ctccacagac agatttgagg 181 ggaactttgt tcacggagaa aagaacggac gggggaagtt cttcttcttt gatggcagca 241 ccctggaggg gtattatgtg gatgatgcct tgcagggcca gggagtttac acttacgaag 301 atgggggagt tctccagggc acgtatgtag acggagagct gaacggtcca gcccaggaat 361 atgacacaga tgggagactg atcttcaagg ggcagtataa agataacatt cgtcatggag 421 tgtgctggat atattaccca gatggaggaa gccttgtagg agaagtaaat gaagatgggg 481 agatgactgg agagaagata gcctatgtgt accctgatga gaggaccgca ctttatggga 541 aatttattga tggagagatg atagaaggca aactggctac ccttatgtcc actgaagaag 601 ggaggcctca ctttgaactg atgcctggaa attcagtgta ccactttgat aagtcgactt 661 catcttgcat ttctaccaat gctcttcttc cagatcctta tgaatcagaa agggtttatg 721 ttgctgaatc tcttatttcc agtgctggag aaggactttt ttcaaaggta gctgtgggac 781 ctaatactgt tatgtctttt tataatggag ttcgaattac acaccaagag gttgacagca 841 gggactgggc ccttaatggg aacaccctct cccttgatga agaaacggtc attgatgtgc 901 ctgagcccta taaccacgta tccaagtact gtgcctcctt gggacacaag gcaaatcact 961 ccttcactcc aaactgcatc TACGATATGT TTGTCCACCC CCGTTTTGGG CCCATCAAAT 1021 GCATCCGCAC CCTGAGAGCA GTGGAGGCCG ATGAAGAGCT CACCGTTGCC TATGGCTATG 1081 ACCACAGCCC CCCCGGGAAG AGTGGGCCTG AAGCCCCTGA GTGGTACCAG GTGGAGCTGA 1141 AGGCCTTCCA GGCCACCCAG CAAAAGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 1201 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1261 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCTTCTGG 1321 ATACGAGTGA TTTGTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1381 aagatt