Construct: ORF TRCN0000474651
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017513.1_s317c1
- Derived from:
- ccsbBroadEn_06225
- DNA Barcode:
- AGGTACCGAGCAGATTTAACCCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FTH1 (2495)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474651
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2495 | FTH1 | ferritin heavy chain 1 | NM_002032.3 | 99.6% | 98.9% | 262A>G;293T>A |
| 2 | human | 2498 | FTH1P3 | ferritin heavy chain 1 pseu... | NR_002201.1 | 55% | (many diffs) | |
| 3 | mouse | 14319 | Fth1 | ferritin heavy polypeptide 1 | NM_010239.2 | 89.4% | 90.7% | (many diffs) |
| 4 | mouse | 14319 | Fth1 | ferritin heavy polypeptide 1 | NR_073181.1 | 32.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 615
- ORF length:
- 549
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac gaccgcgtcc acctcgcagg tgcgccagaa ctaccaccag gactcagagg 121 ccgccatcaa ccgccagatc aacctggagc tctacgcctc ctacgtttac ctgtccatgt 181 cttactactt tgaccgcgat gatgtggctt tgaagaactt tgccaaatac tttcttcacc 241 aatctcatga ggagagggaa catgctgaga aactgatgaa gcTGCAGAAC CAACGAGGTG 301 GCCGAATCTT CCTTCAGGAT ATCAAGGAAC CAGACTGTGA TGACTGGGAG AGCGGGCAGA 361 ATGCAATGGA GTGTGCATTA CATTTGGAAA AAAATGTGAA TCAGTCACTA CTGGAACTGC 421 ACAAACTGGC CACTGACAAA AATGACCCCC ATTTGTGTGA CTTCATTGAG ACACATTACC 481 TGAATGAGCA GGTGAAAGCC ATCAAAGAAT TGGGTGACCA CGTGACCAAC TTGCGCAAGA 541 TGGGAGCGCC CGAATCTGGC TTGGCGGAAT ATCTCTTTGA CAAGCACACC CTGGGAGACA 601 GTGATAATGA AAGCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 661 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 721 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AGGTACCGAG CAGATTTAAC 781 CCTAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt