Construct: ORF TRCN0000474658
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000695.1_s317c1
- Derived from:
- ccsbBroadEn_15755
- DNA Barcode:
- AAGTCGACTAGTTTGACGTGATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUP160 (23279)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474658
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23279 | NUP160 | nucleoporin 160 | NM_001318399.1 | 84.8% | 84.8% | 1_102del |
| 2 | human | 23279 | NUP160 | nucleoporin 160 | NM_015231.2 | 12.1% | 10.1% | (many diffs) |
| 3 | human | 23279 | NUP160 | nucleoporin 160 | NR_134636.2 | 9.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 636
- ORF length:
- 570
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggcggga gccctggaac ggagcttcgt ggagctaagc ggagctgagc 121 gcgaaaggcc gaggcacttt cgggaattca cagtctgcag cattgggact gcaaatgccg 181 tggctGGCGC CGTAAAATAC AGTGAAAGCG CGGGAGGCTT TTACTACGTG GAGAGTGGCA 241 AGTTGTTCTC CGTAACCAGA AACAGGTTCA TTCATTGGAA GACCTCTGGA GATACATTGG 301 AGCTGATGGA GGAGTCACTG GACATAAATC TGTTGAATAA TGCCATTCGC CTAAAATTCC 361 AAAATTGCAG TGTTTTACCT GGAGGGGTTT ATGTCTCTGA GACTCAGAAT CGTGTGATAA 421 TCTTGATGTT AACCAATCAA ACAGTGCACA GGTTACTTTT ACCACACCCC TCCCGGATGT 481 ATAGGAGTGT AAGTTGGCTA AGTGCAATAT CTTTTATTTC CCAAATTACT CTGGGTGTCA 541 CAAATGTAGT GCTGGAGCGA TGTCTTTTGG AATTGAAGGA AATTTGGATT CTCGTTATCC 601 CTCACCAAGC ATACTTTGAT AGCTACCGCT TAAAATGCCC AACTTTCTTG TACAAAGTGG 661 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 721 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 781 AAAGTCGACT AGTTTGACGT GATTGACGCG TTAAGTCgac aatcaacctc tggattacaa 841 aatttgtgaa agatt