Construct: ORF TRCN0000474671
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005542.1_s317c1
- Derived from:
- ccsbBroadEn_06136
- DNA Barcode:
- CAGTATTGGGGGTACAGAGGCGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ECHS1 (1892)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474671
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1892 | ECHS1 | enoyl-CoA hydratase, short ... | NM_004092.4 | 99.7% | 99.6% | 224C>T;582T>C |
| 2 | human | 1892 | ECHS1 | enoyl-CoA hydratase, short ... | XR_002956965.1 | 41% | (many diffs) | |
| 3 | mouse | 93747 | Echs1 | enoyl Coenzyme A hydratase,... | NM_053119.2 | 84.8% | 87.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 936
- ORF length:
- 870
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgccctgcgt gtcctgctgt cctgcgtccg cggcccgctg aggcccccgg 121 ttcgctgtcc cgcctggcgt cccttcgcct cgggtgctaa ctttgagtac atcatcgcag 181 aaaaaagagg gaagaataac accgtggggt tgatccaact gaaccgcccc aaggccctca 241 atgcactttg cgatggcctg attgacgagc tcaaccaggc cctgaagatc ttcgaggagg 301 acccggccgt gggggccatt gtcctcaccg gcggggataa ggcctttgca gctggagctg 361 atatcaagga aatgcagaac ctgagtttcc aggactgtta ctccagcaag ttcttgaagc 421 actgggacca cctcacccag gtcaagaagc cagtcatcgc tgctgtcaat GGCTATGCCT 481 TTGGCGGGGG CTGTGAGCTT GCCATGATGT GTGATATCAT CTATGCCGGT GAGAAGGCCC 541 AGTTTGCACA GCCGGAGATC TTAATAGGAA CCATCCCAGG TGCGGGCGGC ACCCAGAGAC 601 TCACCCGTGC TGTTGGGAAG TCGCTGGCGA TGGAGATGGT CCTCACCGGT GACCGGATCT 661 CAGCCCAGGA CGCCAAGCAA GCAGGTCTTG TCAGCAAGAT TTGTCCTGTT GAGACACTGG 721 TGGAAGAAGC CATCCAGTGT GCAGAAAAAA TTGCCAGCAA TTCTAAAATT GTAGTAGCGA 781 TGGCCAAAGA ATCAGTGAAT GCAGCTTTTG AAATGACATT AACAGAAGGA AGTAAGTTGG 841 AGAAGAAACT CTTTTATTCA ACCTTTGCCA CTGATGACCG GAAAGAAGGG ATGACCGCGT 901 TTGTGGAAAA GAGAAAGGCC AACTTCAAAG ACCAGTGCCC AACTTTCTTG TACAAAGTGG 961 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1021 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1081 ACAGTATTGG GGGTACAGAG GCGACACGCG TTAAGTCgac aatcaacctc tggattacaa 1141 aatttgtgaa agatt