Transcript: Mouse NM_053119.2

Mus musculus enoyl Coenzyme A hydratase, short chain, 1, mitochondrial (Echs1), mRNA.

Source:
NCBI, updated 2017-05-13
Taxon:
Mus musculus (mouse)
Gene:
Echs1 (93747)
Length:
1491
CDS:
30..902

Additional Resources:

NCBI RefSeq record:
NM_053119.2
NBCI Gene record:
Echs1 (93747)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_053119.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000177368 GAGGCAAATAAATGATCCAAA pLKO.1 955 3UTR 100% 4.950 6.930 N Echs1 n/a
2 TRCN0000297554 GAGGCAAATAAATGATCCAAA pLKO_005 955 3UTR 100% 4.950 6.930 N Echs1 n/a
3 TRCN0000181664 GCTGTCAATGGTTATGCTCTT pLKO.1 426 CDS 100% 4.050 3.240 N Echs1 n/a
4 TRCN0000181986 CCGAACATTTCAGGACTGTTA pLKO.1 344 CDS 100% 4.950 3.465 N Echs1 n/a
5 TRCN0000278810 CCGAACATTTCAGGACTGTTA pLKO_005 344 CDS 100% 4.950 3.465 N Echs1 n/a
6 TRCN0000182628 GCAGCCTTTGAGATGACGTTA pLKO.1 765 CDS 100% 4.950 3.465 N Echs1 n/a
7 TRCN0000278758 GCAGCCTTTGAGATGACGTTA pLKO_005 765 CDS 100% 4.950 3.465 N Echs1 n/a
8 TRCN0000181848 GAATCTGTGAATGCAGCCTTT pLKO.1 753 CDS 100% 4.050 2.835 N Echs1 n/a
9 TRCN0000278809 GAATCTGTGAATGCAGCCTTT pLKO_005 753 CDS 100% 4.050 2.835 N Echs1 n/a
10 TRCN0000198371 GAAATGCAGAACCGAACATTT pLKO.1 333 CDS 100% 1.320 0.924 N Echs1 n/a
11 TRCN0000178365 GCTTTCAAGGTGTCTTGATTT pLKO.1 1116 3UTR 100% 1.320 0.924 N Echs1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_053119.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06136 pDONR223 100% 84.8% 87.2% None (many diffs) n/a
2 ccsbBroad304_06136 pLX_304 0% 84.8% 87.2% V5 (many diffs) n/a
3 TRCN0000474671 CAGTATTGGGGGTACAGAGGCGAC pLX_317 53.4% 84.8% 87.2% V5 (many diffs) n/a
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