Construct: ORF TRCN0000474677
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009598.1_s317c1
- Derived from:
- ccsbBroadEn_00220
- DNA Barcode:
- CCATTCATATTATTATAAGTATAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CASP1 (834)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474677
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 834 | CASP1 | caspase 1 | NM_033293.4 | 100% | 100% | |
| 2 | human | 834 | CASP1 | caspase 1 | NM_033294.3 | 84.5% | 84.5% | 582_583ins144 |
| 3 | human | 834 | CASP1 | caspase 1 | XM_017018395.1 | 81.3% | 77.8% | (many diffs) |
| 4 | human | 834 | CASP1 | caspase 1 | NM_001223.5 | 81.2% | 81.2% | 57_272del |
| 5 | human | 834 | CASP1 | caspase 1 | NM_001257119.3 | 81.2% | 81.2% | 57_272del |
| 6 | human | 834 | CASP1 | caspase 1 | XM_017018396.1 | 79% | 79.1% | (many diffs) |
| 7 | human | 834 | CASP1 | caspase 1 | NM_001257118.3 | 76.9% | 76.9% | 57_335del |
| 8 | human | 834 | CASP1 | caspase 1 | NM_033292.4 | 76.9% | 76.9% | 57_335del |
| 9 | human | 834 | CASP1 | caspase 1 | XM_017018393.1 | 73.8% | 73.8% | (many diffs) |
| 10 | human | 834 | CASP1 | caspase 1 | XM_017018394.1 | 73.8% | 73.8% | (many diffs) |
| 11 | human | 834 | CASP1 | caspase 1 | NM_033295.3 | 24.8% | 23.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 999
- ORF length:
- 933
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgacaaggtc ctgaaggaga agagaaagct gtttatccgt tccatgggtg 121 aagctcctca ggcagtgcag gacaacccag ctatgcccac atcctcaggc tcagaaggga 181 atgtcaagct ttgctcccta gaagaagctc aaaggatatg gaaacaaaag tcggcagaga 241 tttatccaat aatggacaag tcaagccgca cacgtcttgc tctcattatc tgcaatgaag 301 aatttgacag tattcctaga agaactggag ctgaggttga catcacaggc atgacaatgc 361 tgctacaaaa tctggggtac agcgtagatg tgaaaaaaaa tctcactgct tcggacatga 421 ctacagagct ggaggcattt gcacaccgcc cagagcacaa gacctctgac agcacgttcc 481 tggtgttcat gtctcatggt attcgggaag gcatttgtgg gaagaaacac tctgagcaag 541 tcccagatat actacaactc aatgcaatct ttaacatgtt gaataccaag aactgcccaa 601 gtttgaagga caaaccGAAG GTGATCATCA TCCAGGCCTG CCGTGGTGAC AGCCCTGGTG 661 TGGTGTGGTT TAAAGATTCA GTAGGAGTTT CTGGAAACCT ATCTTTACCA ACTACAGAAG 721 AGTTTGAGGA TGATGCTATT AAGAAAGCCC ACATAGAGAA GGATTTTATC GCTTTCTGCT 781 CTTCCACACC AGATAATGTT TCTTGGAGAC ATCCCACAAT GGGCTCTGTT TTTATTGGAA 841 GACTCATTGA ACATATGCAA GAATATGCCT GTTCCTGTGA TGTGGAGGAA ATTTTCCGCA 901 AGGTTCGATT TTCATTTGAG CAGCCAGATG GTAGAGCGCA GATGCCCACC ACTGAAAGAG 961 TGACTTTGAC AAGATGTTTC TACCTCTTCC CAGGACATTA CCCAACTTTC TTGTACAAAG 1021 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1081 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1141 ACGACCATTC ATATTATTAT AAGTATAAAC GCGTTAAGTC gacaatcaac ctctggatta 1201 caaaatttgt gaaagatt