Construct: ORF TRCN0000474716
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003961.1_s317c1
- Derived from:
- ccsbBroadEn_11031
- DNA Barcode:
- GCCTTCTTTCCCTATATGTTCTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIK3R2 (5296)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474716
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5296 | PIK3R2 | phosphoinositide-3-kinase r... | NM_005027.4 | 34.7% | 34.7% | 1_1425del;1911T>C |
| 2 | human | 5296 | PIK3R2 | phosphoinositide-3-kinase r... | NR_162071.1 | 20.1% | (many diffs) | |
| 3 | human | 5296 | PIK3R2 | phosphoinositide-3-kinase r... | NR_073517.2 | 18.8% | 1_2029del;2515T>C;2789_4029del | |
| 4 | mouse | 18709 | Pik3r2 | phosphoinositide-3-kinase r... | XM_006509600.3 | 33.6% | 37.2% | (many diffs) |
| 5 | mouse | 18709 | Pik3r2 | phosphoinositide-3-kinase r... | NM_008841.3 | 31% | 34.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 825
- ORF length:
- 759
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gcgtactgca attgaggcct tcaatgagac tatcaagatc tttgaagagc 121 agggccagac tcaagagaaa tgcagcaagg aatacctgga gcgcttccgg cgtgagggca 181 acgagaaaga gatgcaaagg atcctgctga actccgagcg gctcaagtcc cgcattgccg 241 agatccatga gagccgcacg aagctggagc agcagctgcg ggcccaggcc tcggacaaca 301 gagagatcga caagcgcatg aacagcctca agccggacct catgcagctg cgcaagatcc 361 gagaccagta ccTCGTGTGG CTCACCCAGA AAGGCGCCCG GCAGAAGAAA ATCAACGAGT 421 GGCTGGGGAT TAAAAATGAG ACTGAGGACC AGTACGCACT CATGGAGGAC GAGGACGATC 481 TCCCGCACCA CGAGGAACGC ACTTGGTACG TGGGCAAGAT CAACCGCACG CAGGCAGAGG 541 AGATGCTGAG CGGCAAGCGG GATGGCACCT TCCTCATCCG CGAGAGCAGC CAGCGGGGCT 601 GCTACGCCTG CTCCGTGGTA GTGGACGGCG ACACCAAGCA CTGCGTCATC TACCGCACGG 661 CCACCGGCTT CGGCTTCGCG GAGCCCTACA ACCTGTACGG GTCGCTGAAG GAGCTGGTGC 721 TGCACTACCA GCACGCCTCG CTGGTGCAGC ACAACGACGC GCTCACCGTC ACCCTGGCGC 781 ACCCAGTGCG CGCCCCGGGC CCCGGCCCGC CGCCTGCCGC CCGCTGCCCA ACTTTCTTGT 841 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 901 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 961 GAAAGGACGA GCCTTCTTTC CCTATATGTT CTATACGCGT TAAGTCgaca atcaacctct 1021 ggattacaaa atttgtgaaa gatt