Construct: ORF TRCN0000474718
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014288.1_s317c1
- Derived from:
- ccsbBroadEn_15751
- DNA Barcode:
- ATAGGCCCGGCGTTACGGTCCACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSME4 (23198)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474718
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23198 | PSME4 | proteasome activator subunit 4 | XM_006711969.1 | 21.4% | 21.4% | 1_2358del |
| 2 | human | 23198 | PSME4 | proteasome activator subunit 4 | NM_014614.3 | 11.6% | 11.6% | 1_4884del |
| 3 | human | 23198 | PSME4 | proteasome activator subunit 4 | XM_011532707.2 | 11.6% | 11.6% | 1_4884del;5263_5274del |
| 4 | human | 23198 | PSME4 | proteasome activator subunit 4 | XM_011532706.2 | 11.5% | 11.5% | 1_4950del |
| 5 | human | 23198 | PSME4 | proteasome activator subunit 4 | XM_011532705.2 | 11.5% | 11.5% | 1_4950del;5329_5340del |
| 6 | human | 23198 | PSME4 | proteasome activator subunit 4 | XR_001738679.1 | 6.7% | 1_4964del;5178_5179ins163;5447_6958del | |
| 7 | mouse | 103554 | Psme4 | proteasome (prosome, macrop... | NM_134013.3 | 10.7% | 11.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 711
- ORF length:
- 645
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tcaggggttg ctttaccctc atcaagtgcc tttggtactt caggtgctaa 121 aacaaacagc aagaagcagt tcttggcatg cacgatacac agtactgacc tacctccaga 181 ccatggtatt ttataacctc tttattttcc taaacaatga agatgcagtt aaagatatca 241 ggtggctggt tataagtctt ttggaggacg aacaactgga ggttcgagaa atggctgcta 301 ctaccttaag cggTCTGCTA CAGTGTAACT TTCTTACCAT GGACAGTCCT ATGCAGATTC 361 ATTTTGAGCA ACTTTGCAAA ACAAAACTAC CTAAGAAAAG AAAGCGAGAC CCTGGTTCTG 421 TAGGAGATAC CATTCCTTCT GCAGAGTTGG TCAAACGCCA TGCTGGGGTG CTAGGACTTG 481 GTGCATGTGT TCTTTCTAGT CCTTACGATG TTCCCACCTG GATGCCCCAG CTCCTCATGA 541 ATCTCAGTGC ACATCTAAAT GATCCTCAGC CTATTGAGAT GACTGTAAAA AAAACCTTAT 601 CCAATTTCCG AAGGACTCAC CATGACAACT GGCAGGAACA TAAACAGCAA TTCACTGATG 661 ACCAACTGCT TGTTCTCACC GATCTTCTTG TGTCACCATG CTATTATGCA TACCCAACTT 721 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 781 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 841 CTTGTGGAAA GGACGAATAG GCCCGGCGTT ACGGTCCACG ACGCGTTAAG TCgacaatca 901 acctctggat tacaaaattt gtgaaagatt