Construct: ORF TRCN0000474734
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011880.1_s317c1
- Derived from:
- ccsbBroadEn_07058
- DNA Barcode:
- CTTAGTCTTCGACATTTGTTCATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TGFBI (7045)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474734
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7045 | TGFBI | transforming growth factor ... | NM_000358.3 | 99.8% | 100% | (many diffs) |
| 2 | mouse | 21810 | Tgfbi | transforming growth factor,... | NM_009369.4 | 86.9% | 90.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2115
- ORF length:
- 2049
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gctcttcgtg cggctgctgg ctctcgccct ggctctggcc ctgggccccg 121 ccgcgaccct ggcgggtccc gccaagtcgc cctaccagct ggtgctgcag cacagcaggc 181 tccggggccg ccagcacggc cccaacgtgt gtgctgtgca gaaggttatt ggcactaata 241 ggaagtactt caccaactgc aagcagtggt accaaaggaa aatctgtggc aaatcaacag 301 tcatcagcta cgagtgctgt cctggatatg aaaaggtccc tggggagaag ggctgtccag 361 cagccctacc actctcaaac ctttacgaga ccctgggagt cgttggatcc accaccactc 421 agctgtacac ggaccgcacg gagaagctga ggcctgagat ggaggggccc ggcagcttca 481 ccatcttcgc ccctagcaac gaggcctggg cctccttgcc agctgaagtg ctggactccc 541 tggtcagcaa tgtcaacatt gagctgctca atgccctccg ctaccatatg gtgggcaggc 601 gagtcctgac tgatgagctg aaacacggca tgaccctcac ctctatgtac cagaattcca 661 acatccagat ccaccactat cctaatggga ttgtaactgt gaactgtgcc cggctgctga 721 aagccgacca ccatgcaacc aacggggtgg tgcacctcat cgataaggtc atctccacca 781 tcaccaacaa catccagcag atcattgaga tcgaggacac ctttgagacc cttcgggctg 841 ctgtggctgc atcagggctc aacacgatgc ttgaaggcaa cggccagtac acgcttttgg 901 ccccgaccaa tgaggccttc gagaagatcc ctagtgagac tttgaaccgt atcctgggcg 961 acccagaagc cctgagagac ctgctgaaca accacatctt gaagtcagct atgtgtgctg 1021 aagccatcgt tgcggggctg tctgtggaga ccctggaggg cacgacactg gaggtgggct 1081 gcagcgggga catgctcact atcaacggga aggcgatcat ctccaataaa gacatcctag 1141 ccaccaacgg ggtgatccac tacattgatg agctactcat cccagactca gccaagacac 1201 tatttgaatt ggctgcagag tctgatgtgt ccacagccat tgaccttttc agacaagccg 1261 gcctcggcaa tcatctctct ggaagtgagc ggttgaccct cctggctccc ctgaattctg 1321 tattcaaaga tggaacccct ccaattgatg cccatacaag gaatttgctt cggaaccaca 1381 taattaaaga ccagctggcc tctaagtatc tgtaccatgg acagaccctg gaaactctgg 1441 gcggcaaaaa actgagagtt tttgtttatc gtaatagcct ttgcattgag aacagctgca 1501 tcgcggccca cgacaagagg gggaggtacg ggaccctgtt cacgatggac cgggtgctga 1561 cccccccaat ggggactgtc atggatgtcc tgaagggaga caatcgcttt agcatgctgg 1621 tagctgccat ccagtctgca ggactgacgg agaccctcaa ccgggaagga gtctacacag 1681 tcttcgctcc cacaaatgaa gccttccgag ccctgccacc aagagaacgg agcagactct 1741 tgggagatgc caaggaactt gccaacatcc tgaaatacca cattggtgat gaaatcctgg 1801 ttagcggagg catcggggcc ctggtgcggc taaagtctct ccaaggtgac aagctggaag 1861 tcagcttgaa aaacaatgtg gtgagtgtca acaaggagcc tgttgccgag cctgacatca 1921 tggccacaaa tggcgtggtc catgtcatca ccaatgttcT GCAGCCTCCA GCCAACAGAC 1981 CTCAGGAAAG AGGGGATGAA CTTGCAGACT CTGCGCTTGA GATCTTCAAA CAAGCATCAG 2041 CGTTTTCCAG GGCTTCCCAG AGGTCTGTGC GACTAGCCCC TGTCTATCAA AAGTTATTAG 2101 AGAGGATGAA GCATTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2161 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2221 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTTAGTCTTC GACATTTGTT 2281 CATTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt