Construct: ORF TRCN0000474734

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011880.1_s317c1
Derived from:
ccsbBroadEn_07058
DNA Barcode:
CTTAGTCTTCGACATTTGTTCATT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TGFBI (7045)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474734
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7045 TGFBI transforming growth factor ... NM_000358.3 99.8% 100% (many diffs)
2 mouse 21810 Tgfbi transforming growth factor,... NM_009369.4 86.9% 90.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2115
ORF length:
2049
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc gctcttcgtg cggctgctgg ctctcgccct ggctctggcc ctgggccccg
121ccgcgaccct ggcgggtccc gccaagtcgc cctaccagct ggtgctgcag cacagcaggc
181tccggggccg ccagcacggc cccaacgtgt gtgctgtgca gaaggttatt ggcactaata
241ggaagtactt caccaactgc aagcagtggt accaaaggaa aatctgtggc aaatcaacag
301tcatcagcta cgagtgctgt cctggatatg aaaaggtccc tggggagaag ggctgtccag
361cagccctacc actctcaaac ctttacgaga ccctgggagt cgttggatcc accaccactc
421agctgtacac ggaccgcacg gagaagctga ggcctgagat ggaggggccc ggcagcttca
481ccatcttcgc ccctagcaac gaggcctggg cctccttgcc agctgaagtg ctggactccc
541tggtcagcaa tgtcaacatt gagctgctca atgccctccg ctaccatatg gtgggcaggc
601gagtcctgac tgatgagctg aaacacggca tgaccctcac ctctatgtac cagaattcca
661acatccagat ccaccactat cctaatggga ttgtaactgt gaactgtgcc cggctgctga
721aagccgacca ccatgcaacc aacggggtgg tgcacctcat cgataaggtc atctccacca
781tcaccaacaa catccagcag atcattgaga tcgaggacac ctttgagacc cttcgggctg
841ctgtggctgc atcagggctc aacacgatgc ttgaaggcaa cggccagtac acgcttttgg
901ccccgaccaa tgaggccttc gagaagatcc ctagtgagac tttgaaccgt atcctgggcg
961acccagaagc cctgagagac ctgctgaaca accacatctt gaagtcagct atgtgtgctg
1021aagccatcgt tgcggggctg tctgtggaga ccctggaggg cacgacactg gaggtgggct
1081gcagcgggga catgctcact atcaacggga aggcgatcat ctccaataaa gacatcctag
1141ccaccaacgg ggtgatccac tacattgatg agctactcat cccagactca gccaagacac
1201tatttgaatt ggctgcagag tctgatgtgt ccacagccat tgaccttttc agacaagccg
1261gcctcggcaa tcatctctct ggaagtgagc ggttgaccct cctggctccc ctgaattctg
1321tattcaaaga tggaacccct ccaattgatg cccatacaag gaatttgctt cggaaccaca
1381taattaaaga ccagctggcc tctaagtatc tgtaccatgg acagaccctg gaaactctgg
1441gcggcaaaaa actgagagtt tttgtttatc gtaatagcct ttgcattgag aacagctgca
1501tcgcggccca cgacaagagg gggaggtacg ggaccctgtt cacgatggac cgggtgctga
1561cccccccaat ggggactgtc atggatgtcc tgaagggaga caatcgcttt agcatgctgg
1621tagctgccat ccagtctgca ggactgacgg agaccctcaa ccgggaagga gtctacacag
1681tcttcgctcc cacaaatgaa gccttccgag ccctgccacc aagagaacgg agcagactct
1741tgggagatgc caaggaactt gccaacatcc tgaaatacca cattggtgat gaaatcctgg
1801ttagcggagg catcggggcc ctggtgcggc taaagtctct ccaaggtgac aagctggaag
1861tcagcttgaa aaacaatgtg gtgagtgtca acaaggagcc tgttgccgag cctgacatca
1921tggccacaaa tggcgtggtc catgtcatca ccaatgttcT GCAGCCTCCA GCCAACAGAC
1981CTCAGGAAAG AGGGGATGAA CTTGCAGACT CTGCGCTTGA GATCTTCAAA CAAGCATCAG
2041CGTTTTCCAG GGCTTCCCAG AGGTCTGTGC GACTAGCCCC TGTCTATCAA AAGTTATTAG
2101AGAGGATGAA GCATTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
2161CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
2221TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTTAGTCTTC GACATTTGTT
2281CATTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)