Construct: ORF TRCN0000474751
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010175.1_s317c1
- Derived from:
- ccsbBroadEn_11817
- DNA Barcode:
- CGCTCATGGTCAGTGCTAGTTTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF658 (26149)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474751
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26149 | ZNF658 | zinc finger protein 658 | NR_134255.1 | 82% | (many diffs) | |
| 2 | human | 26149 | ZNF658 | zinc finger protein 658 | NM_001317916.1 | 56.9% | 56.2% | (many diffs) |
| 3 | human | 26149 | ZNF658 | zinc finger protein 658 | NM_033160.6 | 56.9% | 56.2% | (many diffs) |
| 4 | human | 26149 | ZNF658 | zinc finger protein 658 | XM_005272515.5 | 56.9% | 56.2% | (many diffs) |
| 5 | human | 26149 | ZNF658 | zinc finger protein 658 | XM_011545679.3 | 56.9% | 56.2% | (many diffs) |
| 6 | human | 26149 | ZNF658 | zinc finger protein 658 | XM_017014614.1 | 56.9% | 56.2% | (many diffs) |
| 7 | human | 401509 | ZNF658B | zinc finger protein 658B (p... | NR_003528.3 | 44.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1911
- ORF length:
- 1845
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tcaggcatca gtgtcattcc aggacgtgac tgtggaattc acccgggagg 121 agtggcagca cctgggccct gtcgagagga cgctgtacag agatgtgatg ctggagaact 181 acagccacct catctcagtg ggatattgca ttactaaacc caaggtgatc tccaagttgg 241 agaaaggaga agagccatgg tctttagaag atgaattcct gaaccagagg tacccaggat 301 attttaaagt tgatcacatc aaagggatcc gggaaaaaca agaaaaacct ctgtggcaag 361 aaatattcat cagtgatgct gacaaaacat tgagtaaaga aggacagaaa gttttagaaa 421 aaccatttaa tctggaaata gctccagagc tttcagaaaa aatatcctgt aaatgtgact 481 cacacagaat gaatttgcca gttgcttctc aattaattat aagtgaaaga aaatattcaa 541 gaaagaagac tgaatacatg aatgtgtgtg agaaactgca gcttgatatt aagcatgaga 601 aagctcatgc tgaagagaaa tcttatgaac atggtgaaaa tgctaaagct ttcagttata 661 agaaagatca gcattggaaa tttcaaactt tggaggaatc ttttgaatgt gatggatctg 721 gacaaggttt atatgataag acaatttgta ttacacctca gagttttcta acaggagaaa 781 agtcctgtaa ggatgatgaa tttagaaaaa actttgataa aatcacttta tttaaccaca 841 tgagaactga cacaaggggg aaatgctctg atcttaatga atatgggaca tcctgtgaca 901 aaaccaccgc tgttgaatac aataaagttc acatggctat gacacactat gagtgtaatg 961 aaagggggat taatttcagt aggaagtcac ccctcactca atctcagaga actattacag 1021 gatggagtgc ttttgaaagc aataaatgtg aagaaaattt tagccagagc tcagcccata 1081 tagtacatca gaaaacacaa gctggagata aatttggtga acataatgaa tgtacagatg 1141 ccctctacca gaaattagac tttacagcac atcagagaat tcacacagaa gataaattct 1201 acctttctga tgaacatggg aaatgcagaa aatcctttta ccggaaagca cacctcattc 1261 agcatcagag gccccactca ggagagaaaa cttaccaata tgaggaatgt gcaaaatcct 1321 tttgttcaag ttcacatcct attcagcatc ctggaactta tgtgggattc aaactttatg 1381 aatgtaatga atgtgggaaa gctttctgtc agaattcaaa cctcagtaaa catctgagaa 1441 ttcacacaaa agagaaacct tgtgataaca atggctgtgg gagatcTTAC AAGTCACCCC 1501 TCATAGGACA CCAGAAAACA GATGCAGAGA TGGAACTCTG TGGTGGCAGT GAATATGGGA 1561 AGACATCACA TCTCAAAGGA CATCAGAGAA TTCTCATGGG GGAGAAACCC TATGAATGTA 1621 TTGAATGTGG GAAAACTTTC TCCAAGACAT CACATCTCAG AGCACATCAG AGAATTCACA 1681 CAGGTGAAAA ACCCTATGAA TGTGTTGAAT GTGAGAAAAC TTTCTCTCAC AAGACACACC 1741 TCAGTGTACA TCAGAGAGTT CACACAGGGG AGAAACCCTA TGAATGTAAT GACTGTGGGA 1801 AATCTTTTAC CTATAACTCA GCCCTGAGAG CACATCAAAG AATTCACACA GGGCTTGCTG 1861 TCTGTCATGT TGGTATCTTA ATGGCTACCA AGAGTCTGTT TCATCAGTCG TACCCAACTT 1921 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1981 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2041 CTTGTGGAAA GGACGACGCT CATGGTCAGT GCTAGTTTGA ACGCGTTAAG TCgacaatca 2101 acctctggat tacaaaattt gtgaaagatt