Construct: ORF TRCN0000474754
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017409.1_s317c1
- Derived from:
- ccsbBroadEn_11377
- DNA Barcode:
- AGAGATTATCGATATAAAGGTTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADAMTS4 (9507)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474754
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9507 | ADAMTS4 | ADAM metallopeptidase with ... | NM_005099.6 | 39.8% | 39.1% | (many diffs) |
| 2 | human | 9507 | ADAMTS4 | ADAM metallopeptidase with ... | NM_001320336.2 | 39.4% | 38.7% | (many diffs) |
| 3 | human | 9507 | ADAMTS4 | ADAM metallopeptidase with ... | XR_001737548.2 | 17.6% | (many diffs) | |
| 4 | human | 9507 | ADAMTS4 | ADAM metallopeptidase with ... | XR_001737549.2 | 17% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1083
- ORF length:
- 1017
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ccagacaggc tcgcatcccg ggaggggctt ggcagggcgc tggctgtggg 121 gagcccaacc ctgcctcctg ctccccattg tgccgctctc ctggctggtg tggctgcttc 181 tgctactgct ggcctctctc ctgccctcag cccggctggc cagccccctc ccccgggagg 241 aggagatcgt gtttccagag aagctcaacg gcagcgtcct gcctggctcg ggcgcccctg 301 ccaggctgtt gtgccgcttg caggcctttg gggagacgct gctactagag ctggagcagg 361 actccggtgt gcaggtcgag gggctgacag tgcagtacct gggccaggcg cctgagctgc 421 tgggtggagc agagcctggc acctacctga ctggcaccat caatggagat ccggagtcag 481 tggcatctct gcactgggat gggggagccc tgttaggcgt gttacaatat cggggggctg 541 aactccacct ccagcccctg gagggaggca cccctaactc tgctggggga cctggggctc 601 acatcctacg ccggaagagt cctgccagcg gtcaaggtcc catgtgcaac gtcaaggctc 661 cTCTTGGAAG CCCCAGCCCC AGACCCCGAA GAGCCAAGCG CTTTGCTTCA CTGAGTAGAT 721 TTGTGGAGAC ACTGGTGGTG GCAGATGACA AGATGGCCGC ATTCCACGGT GCGGGGCTAA 781 AGCGCTACCT GCTAACAGTG ATGGCAGCAG CAGCCAAGGC CTTCAAGCAC CCAAGCATCC 841 GCAATCCTGT CAGCTTGGTG GTGACTCGGC TAGTGATCCT GGGGTCAGGC GAGGAGGGGC 901 CCCAAGTGGG GCCCAGTGCT GCCCAGACCC TGCGCAGCTT CTGTGCCTGG CAGCGGGGCC 961 TCAACACCCC TGAGGACTCG GACCCTGACC ACTTTGACAC AGCCATTCTG TTTACCCGTC 1021 AGGTGAGGCC CCAGTCAGCA CCGCAGGCCA TGCACTGCAC AATTCTAAGA AGCGCCACTA 1081 CATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1141 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1201 GGCTTTATAT ATCTTGTGGA AAGGACGAAG AGATTATCGA TATAAAGGTT CCACGCGTTA 1261 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt