Construct: ORF TRCN0000474761
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004836.1_s317c1
- Derived from:
- ccsbBroadEn_02137
- DNA Barcode:
- TGACTTTTACCGTCATAATCGTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRIP13 (9319)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474761
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9319 | TRIP13 | thyroid hormone receptor in... | NM_004237.4 | 100% | 100% | |
| 2 | human | 9319 | TRIP13 | thyroid hormone receptor in... | XM_011514163.2 | 100% | 100% | |
| 3 | human | 9319 | TRIP13 | thyroid hormone receptor in... | NM_001166260.2 | 66.8% | 66.8% | 867_868ins429 |
| 4 | mouse | 69716 | Trip13 | thyroid hormone receptor in... | NM_027182.2 | 87.5% | 93.2% | (many diffs) |
| 5 | mouse | 69716 | Trip13 | thyroid hormone receptor in... | XR_873456.2 | 48.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1362
- ORF length:
- 1296
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cgaggccgtg ggcgacctga agcaggcgct tccctgtgtg gccgagtcgc 121 caacggtcca cgtggaggtg catcagcgcg gcagcagcac tgcaaagaaa gaagacataa 181 acctgagtgt tagaaagcta ctcaacagac ataatattgt gtttggtgat tacacatgga 241 ctgagtttga tgaacctttt ttgaccagaa atgtgcagtc tgtgtctatt attgacacag 301 aattaaaggt taaagactca cagcccatcg atttgagtgc atgcactgtt gcacttcaca 361 ttttccagct gaatgaagat ggccccagca gtgaaaatct ggaggaagag acagaaaaca 421 taattgcagc aaatcactgg gttctacctg cagctgaatt ccatgggctt tgggacagct 481 tggtatacga tgtggaagtc aaatcccatc tcctcgatta tgtgatgaca actttactgt 541 tttcagacaa gaacgtcaac agcaacctca tcacctggaa ccgggtggtg ctgctccacg 601 gtcctcctgg cactggaaaa acatccctgt gtaaagcgtt agcccagaaa ttgacaatta 661 gactttcaag caggtaccga tatggccaat taattgaaat aaacagccac agcctctttt 721 ctaagtggtt ttcggaaagt ggcaagctgg taaccaagat gtttcagaag attcaggatt 781 tgattgatga taaagacgcc ctggtgttcg tgctgattga tgaggtggag agtctcacag 841 ccgcccgaaa tgcctgcagg gcgggcaccg agccatcaga tgccatcCGC GTGGTCAATG 901 CTGTCTTGAC CCAAATTGAT CAGATTAAAA GGCATTCCAA TGTTGTGATT CTGACCACTT 961 CTAACATCAC CGAGAAGATC GACGTGGCCT TCGTGGACAG GGCTGACATC AAGCAGTACA 1021 TTGGGCCACC CTCTGCAGCA GCCATCTTCA AAATCTACCT CTCTTGTTTG GAAGAACTGA 1081 TGAAGTGTCA GATCATATAC CCTCGCCAGC AGCTGCTGAC CCTCCGAGAG CTAGAGATGA 1141 TTGGCTTCAT TGAAAACAAC GTGTCAAAAT TGAGCCTTCT TTTGAATGAC ATTTCAAGGA 1201 AGAGCGAGGG CCTCAGCGGC CGGGTCCTGA GAAAACTCCC CTTTCTGGCT CATGCGCTGT 1261 ATGTCCAGGC CCCCACCGTC ACCATAGAGG GGTTCCTCCA GGCCCTGTCT CTGGCAGTGG 1321 ACAAGCAGTT TGAAGAGAGA AAGAAGCTTG CAGCTTACAT CTGCCCAACT TTCTTGTACA 1381 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1441 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1501 AGGACGATGA CTTTTACCGT CATAATCGTC AACGCGTTAA GTCgacaatc aacctctgga 1561 ttacaaaatt tgtgaaagat t