Construct: ORF TRCN0000474773
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018300.1_s317c1
- Derived from:
- ccsbBroadEn_03004
- DNA Barcode:
- AATATGACGCAACCTATCTCTCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IL17B (27190)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474773
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27190 | IL17B | interleukin 17B | NM_014443.3 | 100% | 100% | |
| 2 | human | 27190 | IL17B | interleukin 17B | XM_017009346.1 | 71.8% | 71.4% | (many diffs) |
| 3 | human | 27190 | IL17B | interleukin 17B | NM_001317987.1 | 71.1% | 71.1% | 0_1ins156 |
| 4 | human | 27190 | IL17B | interleukin 17B | XM_011537622.2 | 71.1% | 71.1% | 0_1ins156 |
| 5 | human | 27190 | IL17B | interleukin 17B | XM_017009347.1 | 71.1% | 71.1% | 0_1ins156 |
| 6 | human | 27190 | IL17B | interleukin 17B | XM_017009348.1 | 71.1% | 71.1% | 0_1ins156 |
| 7 | mouse | 56069 | Il17b | interleukin 17B | NM_019508.1 | 84.8% | 88.3% | (many diffs) |
| 8 | mouse | 56069 | Il17b | interleukin 17B | XM_006526108.3 | 59.5% | 62.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 609
- ORF length:
- 540
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggactggcct cacaacctgc tgtttcttct taccatttcc atcttccTGG 121 GGCTGGGCCA GCCCAGGAGC CCCAAAAGCA AGAGGAAGGG GCAAGGGCGG CCTGGGCCCC 181 TGGCCCCTGG CCCTCACCAG GTGCCACTGG ACCTGGTGTC ACGGATGAAA CCGTATGCCC 241 GCATGGAGGA GTATGAGAGG AACATCGAGG AGATGGTGGC CCAGCTGAGG AACAGCTCAG 301 AGCTGGCCCA GAGAAAGTGT GAGGTCAACT TGCAGCTGTG GATGTCCAAC AAGAGGAGCC 361 TGTCTCCCTG GGGCTACAGC ATCAACCACG ACCCCAGCCG TATCCCCGTG GACCTGCCGG 421 AGGCACGGTG CCTGTGTCTG GGCTGTGTGA ACCCCTTCAC CATGCAGGAG GACCGCAGCA 481 TGGTGAGCGT GCCGGTGTTC AGCCAGGTTC CTGTGCGCCG CCGCCTCTGC CCGCCACCGC 541 CCCGCACAGG GCCTTGCCGC CAGCGCGCAG TCATGGAGAC CATCGCTGTG GGCTGCACCT 601 GCATCTTCTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 661 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 721 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAATATG ACGCAACCTA TCTCTCATAC 781 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt